Liu Guiqing, Zheng Xuehong, Long Hailin, Rao Zhongchen, Cao Li, Han Richou
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China.
Insects. 2021 Apr 7;12(4):327. doi: 10.3390/insects12040327.
By employing a culture-dependent and -independent 16S rRNA and ITS gene high-throughput sequencing analyses, comprehensive information was obtained on the gut bacterial and fungal communities in the ghost moth larvae of three different geographic locations from high-altitude on Tibet plateau and from low-altitude laboratory. Twenty-six culturable bacterial species belonging to 21 genera and 14 fungal species belonging to 12 genera were identified from six populations by culture-dependent method. was the most abundant bacterial species from both the wild and laboratory-reared larvae. The most abundant OTUs in the wild ghost moth populations were Carnobacteriaceae, Enterobacteriaceae for bacteria, and Ascomycota and Basidiomycota for fungi. Larval microbial communities of the wild ghost moth from different geographic locations were not significantly different from each other but significant difference in larval microbial community was detected between the wild and laboratory-reared ghost moth. The larval gut of the wild ghost moth was dominated by the culturable . However, that of the laboratory-reared ghost moth exhibited significantly abundant , , , and . Furthermore, the larval gut of the wild ghost moth had a significantly higher abundance of but lower abundance of and than that of the laboratory-reared ghost moth.
通过采用依赖培养和不依赖培养的16S rRNA和ITS基因高通量测序分析，获得了来自青藏高原高海拔地区和低海拔实验室三个不同地理位置的幽灵蛾幼虫肠道细菌和真菌群落的全面信息。通过依赖培养的方法从六个种群中鉴定出属于21个属的26种可培养细菌和属于12个属的14种真菌。 是野生和实验室饲养幼虫中最丰富的细菌种类。野生幽灵蛾种群中最丰富的OTU细菌是肉食杆菌科、肠杆菌科，真菌是子囊菌门和担子菌门。来自不同地理位置的野生幽灵蛾幼虫微生物群落彼此之间没有显著差异，但在野生和实验室饲养的幽灵蛾之间检测到幼虫微生物群落存在显著差异。野生幽灵蛾的幼虫肠道以可培养的 为主。然而，实验室饲养的幽灵蛾幼虫肠道中 、 、 和 的含量显著丰富。此外，野生幽灵蛾的幼虫肠道中 的丰度显著高于实验室饲养的幽灵蛾，而 和 的丰度则较低。
