School of Basic Medicine, Guangdong Jiangmen Chinese Medicine College, Jiangmen, 529000, China.
Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, 523808, China.
Curr Microbiol. 2022 Jun 29;79(8):229. doi: 10.1007/s00284-022-02919-0.
Chinese cordyceps is a well-known fungus-larva complex with medicinal and economic importance. At present the occurrence of Chinese cordyceps has not been fully illuminated. In this study, the microbial diversities of fertilized Thitarodes eggs from sites A (high occurrence rates of Chinese cordyceps), B (low occurrence rates), and C (no Chinese cordyceps) were analyzed using 16S rRNA and ITS gene-sequencing technique. The previous sequencing data of soil from the same sites were conjointly analyzed. The results showed that bacterial communities among the eggs were significantly different. The bacterial diversity and evenness were much higher on site A. Wolbachia was overwhelmingly predominant in the eggs of sites B and C, while Spiroplasma showed preference on site A. The fungal between-group differences in the eggs were not as significant as that of bacteria. Purpureocillium in Cordyceps-related families showed preference on site A. Wolbachia, Spiroplasma, and Purpureocillium were inferred to be closely related to Chinese cordyceps occurrence. Intra-kingdom and inter-kingdom network analyses suggest that closer correlations of microbial communities (especially closer fungal positive correlations) in fertilized eggs might promote Chinese cordyceps occurrence. Besides, metabolic pathway analysis showed that in fertilized eggs or soil the number of bacterial metabolic pathways with significant differences in every comparison between two sites was greater than that of fungi. Collectively, this study provides novel information about the occurrence of Chinese cordyceps, contributing to the large-scale artificial cultivation of Chinese cordyceps.
中国虫草是一种具有药用和经济价值的著名虫菌复合体。目前，中国虫草的发生规律尚未完全阐明。本研究采用 16S rRNA 和 ITS 基因测序技术，分析了 A （中国虫草发生频率高）、B （中国虫草发生频率低）、C （无中国虫草）三个地点受精的白僵蚕卵的微生物多样性，并与同一地点的土壤先前测序数据进行了联合分析。结果表明，卵中的细菌群落存在显著差异。A 点的细菌多样性和均匀度明显较高。B 点和 C 点的卵中以沃尔巴克氏体为主，而 A 点的卵中以螺旋体为主。卵中真菌的组间差异不如细菌显著。与虫草相关科的紫青霉在 A 点表现出偏好。沃尔巴克氏体、螺旋体和紫青霉被推断与中国虫草的发生密切相关。种内和种间网络分析表明，受精卵中微生物群落（尤其是真菌正相关）的相关性越强，越有利于中国虫草的发生。此外，代谢途径分析表明，在受精卵或土壤中，每个地点两两比较中，细菌代谢途径数量的差异显著大于真菌。综上所述，本研究为中国虫草的发生提供了新的信息，有助于中国虫草的大规模人工栽培。
