Engineering Bioprocess and Biotechnology Graduate Program, Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba 81530-000, Brazil.
Microbiology, Parasitology and Pathology Graduate Program, Department of Basic Pathology, Microbiology, Federal University of Paraná, Curitiba 81530-000, Brazil.
Int J Environ Res Public Health. 2021 Apr 6;18(7):3824. doi: 10.3390/ijerph18073824.
SARS-CoV-2 environmental monitoring can track the rate of viral contamination and can be used to establish preventive measures. This study aimed to detect by RT-PCR the presence of SARS-CoV-2 from inert surface samples in public health settings with a literature review about surface contamination and its burden on spread virus. Samples were collected from health settings in Curitiba, Brazil, between July and December 2020. A literature review was conducted using PRISMA. A total of 711 environmental surface samples were collected from outpatient areas, dental units, doctors' offices, COVID-19 evaluation areas, and hospital units, of which 35 (4.9%) were positive for SARS-CoV-2 RNA. The frequency of environmental contamination was higher in primary care units than in hospital settings. The virus was detected on doctors' personal items. Remarkably, the previously disinfected dental chair samples tested positive. These findings agree with those of other studies in which SARS-CoV-2 was found on inanimate surfaces. Detection of SARS-CoV-2 RNA on surfaces in public health settings, including those not meant to treat COVID-19, indicates widespread environmental contamination. Therefore, the intensification of disinfection measures for external hospital areas may be important for controlling community COVID-19 dissemination.
SARS-CoV-2 环境监测可以跟踪病毒污染率，并可用于制定预防措施。本研究旨在通过 RT-PCR 检测巴西库里提巴公共卫生环境中惰性表面样本中 SARS-CoV-2 的存在情况，并对表面污染及其对病毒传播负担的相关文献进行综述。该研究于 2020 年 7 月至 12 月期间在健康环境中采集样本。采用 PRISMA 进行文献综述。共采集了来自门诊区、牙科单位、医生办公室、COVID-19 评估区和医院单位的 711 个环境表面样本，其中 35 个（4.9%）样本的 SARS-CoV-2 RNA 呈阳性。初级保健单位的环境污染频率高于医院环境。病毒在医生的个人物品上被检测到。值得注意的是，之前已消毒的牙科椅样本检测呈阳性。这些发现与其他研究一致，表明在无生命表面上发现了 SARS-CoV-2。在包括非 COVID-19 治疗单位在内的公共卫生环境中检测到 SARS-CoV-2 RNA 表明存在广泛的环境污染。因此，强化对外医院区的消毒措施可能对控制社区 COVID-19 传播很重要。
