The Centre for Innovation Competence (ZIK) Plasmatis, Leibniz Institute for Plasma Science and Technology (INP), 17489 Greifswald, Germany.
Institute of Hygiene and Environmental Medicine, Greifswald University Medical Center, 17475 Greifswald, Germany.
Int J Mol Sci. 2021 Apr 6;22(7):3790. doi: 10.3390/ijms22073790.
Cold physical plasma is a partially ionized gas expelling many reactive oxygen and nitrogen species (ROS/RNS). Several plasma devices have been licensed for medical use in dermatology, and recent experimental studies suggest their putative role in cancer treatment. In cancer therapies with an immunological dimension, successful antigen presentation and inflammation modulation is a key hallmark to elicit antitumor immunity. Dendritic cells (DCs) are critical for this task. However, the inflammatory consequences of DCs following plasma exposure are unknown. To this end, human monocyte-derived DCs (moDCs) were expanded from isolated human primary monocytes; exposed to plasma; and their metabolic activity, surface marker expression, and cytokine profiles were analyzed. As controls, hydrogen peroxide, hypochlorous acid, and peroxynitrite were used. Among all types of ROS/RNS-mediated treatments, plasma exposure exerted the most notable increase of activation markers at 24 h such as CD25, CD40, and CD83 known to be crucial for T cell costimulation. Moreover, the treatments increased interleukin (IL)-1α, IL-6, and IL-23. Altogether, this study suggests plasma treatment augmenting costimulatory ligand and cytokine expression in human moDCs, which might exert beneficial effects in the tumor microenvironment.
冷等离子体是部分电离的气体，会释放出许多活性氧和氮物种（ROS/RNS）。几种等离子体设备已获得皮肤科医学用途的许可，最近的实验研究表明它们在癌症治疗中有一定的作用。在具有免疫维度的癌症治疗中，成功的抗原呈递和炎症调节是引发抗肿瘤免疫的关键标志。树突状细胞（DC）是完成这项任务的关键。然而，关于 DC 暴露于等离子体后的炎症后果尚不清楚。为此，我们从分离的人原代单核细胞中扩增人单核细胞衍生的树突状细胞（moDC）；使其暴露于等离子体中；并分析其代谢活性、表面标志物表达和细胞因子谱。作为对照，我们使用了过氧化氢、次氯酸和过氧亚硝酸盐。在所有类型的 ROS/RNS 介导的治疗中，暴露于等离子体在 24 小时时可最显著地增加激活标志物，如 CD25、CD40 和 CD83，这些标志物对于 T 细胞共刺激至关重要。此外，这些治疗还增加了白细胞介素（IL）-1α、IL-6 和 IL-23 的表达。总之，这项研究表明，等离子体处理可增强人 moDC 中共刺激配体和细胞因子的表达，这可能对肿瘤微环境产生有益的影响。
