Matthiesen Svea, Jahnke Rico, Knittler Michael R
Institute of Immunology, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, Germany.
Methods Protoc. 2021 Apr 8;4(2):25. doi: 10.3390/mps4020025.
We present a new and straightforward method by which standard cell culture plates can be sealed off from ambient air and be placed under controlled hypoxic cell culture conditions without costly or highly specialized materials. The method was established on a murine cell culture system using the dendritic cell line JAWS II but can be readily adapted to other cell cultures. The procedure was designed to be easy to implement in cell culture laboratories with standard incubators and requires only readily available materials, resources, and consumables, such as six-well plates, degassed culture medium, CoCl, a vacuum sealer, etc., and no further complicated laboratory equipment. The simple hypoxic cell culture method presented here is technically reliable and experimentally safe. As it can be performed in any standard incubator, it is suitable for use at both low and higher biosafety levels.
我们提出了一种全新且简便的方法，通过该方法可将标准细胞培养板与外界空气隔绝，并置于可控的低氧细胞培养条件下，而无需使用昂贵或高度专业化的材料。该方法基于使用树突状细胞系JAWS II的小鼠细胞培养系统建立，但可轻松适用于其他细胞培养。该程序设计为易于在配备标准培养箱的细胞培养实验室中实施，仅需常用的材料、资源和耗材，如六孔板、脱气培养基、CoCl、真空封口机等，无需进一步复杂的实验室设备。此处介绍的简单低氧细胞培养方法在技术上可靠且实验安全。由于它可在任何标准培养箱中进行，适用于低和高生物安全水平的使用。
