Nitz Fabiana, de Melo Bruna Oliveira, da Silva Luís Cláudio Nascimento, de Souza Monteiro Andrea, Marques Sirlei Garcia, Monteiro-Neto Valério, de Jesus Gomes Turri Rosimary, Junior Antonio Dantas Silva, Conceição Patrícia Cristina Ribeiro, Magalhães Hilário José Cardoso, Zagmignan Adrielle, Ferro Thiago Azevedo Feitosa, Bomfim Maria Rosa Quaresma
Laboratório de Biologia Molecular de Microrganismos Patogênicos, Universidade Ceuma, São Luís 65075-120, Brazil.
Laboratório Cedro, São Luís 65020-570, Brazil.
Microorganisms. 2021 Apr 9;9(4):786. doi: 10.3390/microorganisms9040786.
has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among isolates. The drug susceptibility profiles of for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E () gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin ( and ) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of and genes in .
由于具有多重耐药（MDR）特征的菌株出现，已导致高死亡率。本研究旨在对从不同临床标本中分离出的99株菌株的毒力和耐药基因分子特征进行表征。通过Vitek2自动化方法鉴定分离株，并使用不同类别的抗菌药物确定抗生素敏感性特征。提取基因组DNA并通过多重聚合酶链反应（mPCR）进行扩增，以检测不同的毒力和抗菌耐药基因。使用肠杆菌重复基因间共有序列（ERIC-PCR）技术进行分子分型，以确定分离株之间的克隆关系。所有菌株的药敏谱显示出高水平的耐药性，特别是27株（27.3%）表现出广泛耐药（XDR）特征的分离株，其他分离株表现出MDR特征。我们在一株对黏菌素耐药的菌株中检测到多黏菌素E（）基因。赋予对苯唑西林耐药性的基因（和）存在于三株分离株中。其中一株分离株携带这两个基因。据我们从文献中所知，这是首次报道在中存在和基因情况。
