König Elisabeth, Ziegler Hans P, Tribus Julia, Grisold Andrea J, Feierl Gebhard, Leitner Eva
Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, A 8010 Graz, Austria.
Section of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Medical University of Graz, A 8010 Graz, Austria.
Antibiotics (Basel). 2021 Apr 21;10(5):479. doi: 10.3390/antibiotics10050479.
Anaerobic bacteria play an important role in human infections. spp. are some of the 15 most common pathogens causing nosocomial infections. We present antimicrobial susceptibility testing (AST) results of 114 Gram-positive anaerobic isolates and 110 -group-isolates (BFGI). Resistance profiles were determined by MIC gradient testing. Furthermore, we performed disk diffusion testing of BFGI and compared the results of the two methods. Within Gram-positive anaerobes, the highest resistance rates were found for clindamycin and moxifloxacin (21.9% and 16.7%, respectively), and resistance for beta-lactams and metronidazole was low (<1%). For BFGI, the highest resistance rates were also detected for clindamycin and moxifloxacin (50.9% and 36.4%, respectively). Resistance rates for piperacillin/tazobactam and amoxicillin/clavulanic acid were 10% and 7.3%, respectively. Two isolates were classified as multi-drug-resistant (MDR), with resistance against all tested beta-lactam antibiotics. The comparative study of 109 BFGI resulted in 130 discrepancies in 763 readings (17%) with a high number of Very Major Errors (VME) and Major Errors (ME). In summary, resistance rates, with the exception of clindamycin and moxifloxacin, are still low, but we are facing increasing resistance rates for BFGI. Surveillance studies on a regular basis are still recommended.
厌氧菌在人类感染中起重要作用。某些菌种是导致医院感染的15种最常见病原体中的一部分。我们展示了114株革兰氏阳性厌氧菌分离株和110株B群革兰氏阴性菌分离株（BFGI）的抗菌药敏试验（AST）结果。通过MIC梯度试验确定耐药谱。此外，我们对BFGI进行了纸片扩散试验，并比较了两种方法的结果。在革兰氏阳性厌氧菌中，克林霉素和莫西沙星的耐药率最高（分别为21.9%和16.7%），β-内酰胺类和甲硝唑的耐药率较低（<1%）。对于BFGI，克林霉素和莫西沙星的耐药率也最高（分别为50.9%和36.4%）。哌拉西林/他唑巴坦和阿莫西林/克拉维酸的耐药率分别为10%和7.3%。有两株分离株被归类为多重耐药（MDR），对所有测试的β-内酰胺类抗生素均耐药。对109株BFGI的比较研究在763次读数中出现了130处差异（17%），其中有大量的极重大错误（VME）和重大错误（ME）。总之，除克林霉素和莫西沙星外，耐药率仍然较低，但我们面临着BFGI耐药率不断上升的问题。仍建议定期进行监测研究。
