Ikeyama Nao, Sakamoto Mitsuo, Ohkuma Moriya, Hiramoto Shigeru, Wang Jianpeng, Tone Shigenobu, Shiiba Kiwamu
RIKEN BioResource Research Center, Microbe Division/Japan Collection of Microorganisms, Tsukuba, Ibaraki 305-0074, Japan.
Division of Life Science and Engineering, Tokyo Denki University, Ishisaka, Hatoyama, Saitama 350-0394, Japan.
Microorganisms. 2021 Apr 21;9(5):888. doi: 10.3390/microorganisms9050888.
Bamboo hemicellulose hydrolysate (BHH) may possess antihypercholesterolemic activity; however, this activity requires further comprehensive study to assess the prebiotic mechanisms of BHH in vivo. Here, we used high-throughput 16S rRNA gene sequencing to preliminarily investigate the correlations between BHH and the fecal microbiomes of three groups of mice fed either a normal diet, a high-fat diet, or a high-fat diet supplemented with 5% BHH for 5 weeks. Alpha diversity (within community) was nonsignificant for all groups; however, beta diversity analysis among communities showed that 5% BHH suppressed the significant changes induced by the high-fat diet. The / ratio, the family S24-7 within the order , the family and several cellulolytic taxa were slightly ameliorated in the BHH group. These results indicated that BHH supplementation influenced the gut bacterial community and suppressed the high-fat diet-induced alterations. Additionally, BHH significantly lowered the serum cholesterol levels and fecal pH. Improving short-chain fatty acid production for all of the bacterial communities in the mouse guts may induce this effect. Thus, the prebiotic potential of BHH should be evaluated considering the gut microbial communities and their interactions.
竹半纤维素水解物（BHH）可能具有抗高胆固醇活性；然而，这种活性需要进一步全面研究以评估BHH在体内的益生元机制。在此，我们使用高通量16S rRNA基因测序初步研究了BHH与三组分别喂食正常饮食、高脂饮食或补充5%BHH的高脂饮食5周的小鼠粪便微生物群之间的相关性。所有组的α多样性（群落内）无显著差异；然而，群落间的β多样性分析表明，5%BHH抑制了高脂饮食引起的显著变化。BHH组的/比值、目内的S24-7科、科和几个纤维素分解类群略有改善。这些结果表明，补充BHH影响肠道细菌群落并抑制高脂饮食引起的改变。此外，BHH显著降低了血清胆固醇水平和粪便pH值。改善小鼠肠道中所有细菌群落的短链脂肪酸产生可能会导致这种效果。因此，应考虑肠道微生物群落及其相互作用来评估BHH的益生元潜力。
