Sinha D K, White C J
Department of Breast Surgery, Roswell Park Memorial Institute, Buffalo, New York 14263.
In Vitro Cell Dev Biol. 1988 Jun;24(6):525-9. doi: 10.1007/BF02629086.
Collagen gel culture of rat mammary epithelial cells was used as an in vitro assay system for determination of the therapeutic efficacy of three cytotoxic agents commonly used in the treatment of human breast cancer, namely 5-fluorouracil (5-FU), methotrexate, and Adriamyin (ADR). The same three drugs were also evaluated in vivo, and a good correlation was obtained between the results in these two systems. A 9-d culture was shown to be more reliable than a 12-d culture, because nondrug-related cell mortality became a confounding factor after 12 d. Although further experiments are necessary, it is suggested that collagen gel culture may well prove to be a useful assay system for determination of sensitivity of tumor cells to cytotoxic drugs with possible clinical applications in the choice of treatment modality administered to cancer patients.
大鼠乳腺上皮细胞的胶原凝胶培养被用作一种体外测定系统，用于确定三种常用于治疗人类乳腺癌的细胞毒性药物的治疗效果，这三种药物分别是5-氟尿嘧啶（5-FU）、甲氨蝶呤和阿霉素（ADR）。同样的三种药物也在体内进行了评估，并且在这两个系统的结果之间获得了良好的相关性。结果表明，9天的培养比12天的培养更可靠，因为12天后非药物相关的细胞死亡率成为一个混杂因素。尽管还需要进一步的实验，但有人提出，胶原凝胶培养很可能被证明是一种有用的测定系统，用于确定肿瘤细胞对细胞毒性药物的敏感性，在为癌症患者选择治疗方式方面可能具有临床应用价值。
