Li Zhibin, Liu Dasong, Xu Shu, Zhang Wenjin, Zhou Peng
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
International Joint Research Laboratory for Functional Dairy Protein Ingredients, U.S.-China Dairy Innovation Center, Jiangnan University, Wuxi 214122, China.
Foods. 2021 Apr 18;10(4):888. doi: 10.3390/foods10040888.
Effects of pore diameters (100, 50, and 20 nm), concentration factors (1-8) and processing stages (1-5) on the transmission of major serum proteins (β-lactoglobulin and α-lactalbumin) and minor serum proteins (immunoglobulin (Ig) G, IgA, IgM, lactoferrin (LF), lactoperoxidase (LPO), xanthine oxidase (XO)) during ceramic microfiltration (MF) of skim milk were studied. Holstein skim milk was microfiltered at a temperature of 50 °C, a transmembrane pressure of 110 kPa and a crossflow velocity of 6.7 m/s, using a tubular single stainless steel module that consisted of three ceramic tubes, each with 19 channels (3.5 mm inner diameter) and a length of 0.5 m. For MF with 100 nm and 50 nm pore diameters, the recovery yield of major serum proteins in permeate was 44.3% and 44.1%, while the recovery yield of minor serum proteins was slightly less by 0%-8% than 50 nm MF. MF with 20 nm pore diameters showed a markedly lower (by 12%-45%) recovery yield for both major and minor serum proteins, corresponding with its lower membrane flux. Flux sharply decreased with an increasing concentration factor (CF) up to four, and thereafter remained almost unchanged. Compared to the decrease (88%) of flux, the transmission of major and minor serum proteins was decreased by 4%-15% from CF = one to CF = eight. With increasing processing stages, the flux gradually increased, and the recovery yield of both major and minor proteins in the permeate gradually decreased and reached a considerably low value at stage five. After four stages of MF with 100 nm pore diameter and a CF of four for each stage, the cumulative recovery yield of major serum proteins, IgG, IgA, IgM, LF, LPO, and XO reached 95.7%, 90.8%, 68.5%, 34.1%, 15.3%, 39.1% and 81.2% respectively.
研究了孔径（100、50和20纳米）、浓缩因子（1 - 8）和处理阶段（1 - 5）对脱脂乳陶瓷微滤（MF）过程中主要血清蛋白（β-乳球蛋白和α-乳白蛋白）和次要血清蛋白（免疫球蛋白（Ig）G、IgA、IgM、乳铁蛋白（LF）、乳过氧化物酶（LPO）、黄嘌呤氧化酶（XO））传输的影响。荷斯坦脱脂乳在50℃的温度、110 kPa的跨膜压力和6.7 m/s的错流速度下进行微滤，使用由三根陶瓷管组成的管状单不锈钢模块，每根陶瓷管有19个通道（内径3.5毫米），长度为0.5米。对于孔径为100纳米和50纳米的微滤，渗透物中主要血清蛋白的回收率分别为44.3%和44.1%，而次要血清蛋白的回收率比50纳米微滤略低0% - 8%。孔径为20纳米的微滤显示主要和次要血清蛋白的回收率均显著降低（降低12% - 45%），这与其较低的膜通量相对应。通量随着浓缩因子（CF）增加到4而急剧下降，此后几乎保持不变。与通量下降（88%）相比，主要和次要血清蛋白的传输从CF = 1到CF = 8下降了4% - 15%。随着处理阶段的增加，通量逐渐增加，渗透物中主要和次要蛋白的回收率逐渐降低，并在第五阶段达到相当低的值。在孔径为100纳米且每个阶段CF为4的微滤进行四个阶段后，主要血清蛋白、IgG、IgA、IgM、LF、LPO和XO的累积回收率分别达到95.7%、90.8%、68.5%、34.1%、15.3%、39.1%和81.2%。
