Sánchez-Hernández Silvia, Théron Laëtitia, Jiménez-Barrios Pablo, Olalla-Herrera Manuel, Recio Isidra, Miralles Beatriz
Departamento de Nutrición y Bromatología, University of Granada, 18071 Granada, Spain.
Institut National de Recherche Pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), UR370 Qualité des Produits Animaux, F-63122 Saint Genès-Champanelle, France.
Foods. 2021 Apr 18;10(4):887. doi: 10.3390/foods10040887.
Human milk proteins have shown to vary in concentration and distribution through lactation. However, while some regulatory components, such as hormones, have shown associations with regard to the mothers' body mass index, there is limited information on the possible influence of this condition on the whole protein distribution. The objective of this study was to evaluate the protein profile of human milk from normal weight and overweight or obese mothers to identify differences in protein expression in colostrum, transitional and mature milk. The mass spectrometry analysis showed the ability to class with a high degree of confidence the lactation state and the milk profile according to the mother's condition. Individual milk samples were subjected to a digestion in vitro model that takes into account the specificities of the gastrointestinal conditions of full-term newborn infants. The digestion products were compared with available data from the digestive contents in newborns. The behavior of the most abundant proteins and the overall peptide generation and survival, showed good correspondence with in vivo data.
研究表明，人乳蛋白质的浓度和分布会随着哺乳期而变化。然而，虽然一些调节成分（如激素）已显示出与母亲体重指数有关联，但关于这种情况对整体蛋白质分布可能产生的影响，相关信息有限。本研究的目的是评估体重正常和超重或肥胖母亲的人乳蛋白质谱，以确定初乳、过渡乳和成熟乳中蛋白质表达的差异。质谱分析显示，能够根据母亲的状况高度准确地对泌乳状态和乳汁谱进行分类。对个体乳汁样本进行了体外消化模型实验，该模型考虑了足月新生儿胃肠道状况的特殊性。将消化产物与新生儿消化内容物的现有数据进行了比较。最丰富蛋白质的行为以及整体肽的生成和存活情况与体内数据显示出良好的一致性。
