Wang Xiaolian, Lu Dongxiao, Tian Chengming
The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2021 Apr 14;22(8):4029. doi: 10.3390/ijms22084029.
The hemibiotrophic ascomycete fungus is the causal agent of anthracnose on numerous plants, and it causes considerable economic losses worldwide. Endocytosis is an essential cellular process in eukaryotic cells, but its roles in remain unknown. In our study, we identified an endocytosis-related protein, CgEnd3, and knocked it out via polyethylene glycol (PEG)-mediated protoplast transformation. The lack of resulted in severe defects in endocytosis. infects its host through a specialized structure called appressorium, and Δ showed deficient appressorium formation, melanization, turgor pressure accumulation, penetration ability of appressorium, cellophane membrane penetration, and pathogenicity. also affected oxidant adaptation and the expression of core effectors during the early stage of infection. CgEnd3 contains one EF hand domain and four calcium ion-binding sites, and it is involved in calcium signaling. A lack of changed the responses to cell-wall integrity agents and fungicide fludioxonil. However, regulated appressorium formation and endocytosis in a calcium signaling-independent manner. Taken together, these results demonstrate that plays pleiotropic roles in endocytosis, calcium signaling, cell-wall integrity, appressorium formation, penetration, and pathogenicity in , and it suggests that or endocytosis-related genes function as promising antifungal targets.
半活体营养型子囊菌真菌是多种植物炭疽病的病原体，在全球范围内造成了相当大的经济损失。内吞作用是真核细胞中一个重要的细胞过程，但其在……中的作用仍不清楚。在我们的研究中，我们鉴定了一种与内吞作用相关的蛋白CgEnd3，并通过聚乙二醇（PEG）介导的原生质体转化将其敲除。CgEnd3的缺失导致内吞作用出现严重缺陷。……通过一种称为附着胞的特殊结构感染宿主，而CgEnd3缺失突变体（Δ）表现出附着胞形成、黑化、膨压积累、附着胞穿透能力、玻璃纸膜穿透和致病性方面的缺陷。……在感染早期还影响了氧化剂适应性和核心效应子的表达。CgEnd3包含一个EF手型结构域和四个钙离子结合位点，并且参与钙信号传导。CgEnd3的缺失改变了对细胞壁完整性试剂和杀菌剂咯菌腈的反应。然而，……以一种不依赖钙信号传导的方式调节附着胞形成和内吞作用。综上所述，这些结果表明CgEnd3在……的内吞作用、钙信号传导、细胞壁完整性、附着胞形成、穿透和致病性中发挥多效性作用，并且表明CgEnd3或与内吞作用相关的基因可作为有前景的抗真菌靶点。
