Zheng Xiang-Rong, Zhang Mao-Jiao, Qiao Yu-Hang, Li Ran, Alkan Noam, Chen Jie-Yin, Chen Feng-Mao
Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Plant Sci. 2022 Jun 30;13:933484. doi: 10.3389/fpls.2022.933484. eCollection 2022.
is an endemic Chinese tree species with considerable medicinal, timber, and horticultural value. The anthracnose disease of is caused by the fungal pathogen , which results in great losses in yield and quality. Here, resistance evaluation of six cultivars of exhibited varying degrees of resistance to infection, where Wufeng was the most resistant and Jinggangshan was the most susceptive. Physiological measurements and histochemical staining assays showed that the Wufeng cultivar exhibits intense reactive oxygen species accumulation and defense capabilities. A multiomics approach using RNA sequencing and metabolome analyses showed that resistance in (Wufeng) is related to early induction of reprogramming of the flavonoid biosynthesis pathway. antifungal assays revealed that the flavonoid extracts from resistant cultivars strongly inhibited hyphal growth than susceptible cultivars. Relative gene expression analysis further demonstrated the pivotal antifungal role of flavonoids in targeting appressorium formation. Together, these results represent a novel resistance mechanism of against anthracnose through the reprogramming of flavonoids, which will lay a foundation for breeding anthracnose-resistant varieties and the application of flavonoid extraction of as a natural antifungal treatment.
是一种具有重要药用、木材和园艺价值的中国本土树种。的炭疽病由真菌病原体引起，导致产量和品质大幅损失。在此，对六个品种的抗性评估显示出对感染的不同程度抗性，其中五峰最抗病，井冈山最感病。生理测量和组织化学染色分析表明，五峰品种表现出强烈的活性氧积累和防御能力。使用RNA测序和代谢组分析的多组学方法表明，（五峰）的抗性与类黄酮生物合成途径重编程的早期诱导有关。抗真菌试验表明，抗性品种的类黄酮提取物比感病品种更能强烈抑制菌丝生长。相对基因表达分析进一步证明了类黄酮在靶向附着胞形成中起关键抗真菌作用。总之，这些结果代表了通过类黄酮重编程对抗炭疽病的一种新抗性机制，这将为培育抗炭疽病品种以及将类黄酮提取物作为天然抗真菌处理方法的应用奠定基础。
