Yang Xing, Guo Cuimei, Chen Chi, Hu Zhijuan, Zheng Xinyao, Xu Shan, Yang Xingyong, Xie Chengjian
The Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, China.
Chongqing Engineering Research Center of Specialty Crop Resources, The College of Life Science, Chongqing Normal University, Chongqing 401331, China.
J Fungi (Basel). 2022 Apr 12;8(4):391. doi: 10.3390/jof8040391.
The soil-borne vascular fungus infects hundreds of dicotyledonous plants, causing severe wilt diseases. During the initial colonization, develops a penetration peg to enable infection of cotton roots. In some phytopathogenic fungi, vacuoles play a critical role in normal formation of the infection structure. Kinesin 2 protein is associated with vacuole formation in . To identify the function of vacuoles in the infection structure, we identified , an ortholog of kinesin 2, in and investigated its function through gene knockout. mutants showed severe defects in virulence and were suppressed during initial infection and root colonization based on observation of green fluorescent protein-labeled . We also found that deletion of compromised penetration peg formation and the derived septin neck. Disruption strains were viable and showed normal microsclerotia formation, whereas mycelium growth and conidial production were reduced, with shorter and more branched hyphae. Furthermore, the mutant, unlike wild-type , lacked a large basal vacuole, accompanied by a failure to generate concentrated lipid droplets. Taken together, regulates vacuole formation by , which is required for conidiation, mycelium growth, and penetration structure formation during initial plant root infection.
这种土传维管束真菌感染数百种双子叶植物，引发严重的枯萎病。在初始定殖过程中，它会形成一个侵入栓以实现对棉花根部的感染。在一些植物病原真菌中，液泡在感染结构的正常形成中起关键作用。驱动蛋白2蛋白与[具体真菌名称]中的液泡形成有关。为了确定液泡在[具体真菌名称]感染结构中的功能，我们在[具体真菌名称]中鉴定出驱动蛋白2的直系同源物[具体基因名称]，并通过基因敲除研究其功能。基于对绿色荧光蛋白标记的[具体真菌名称]的观察，[具体基因名称]突变体在毒力方面表现出严重缺陷，并且在初始感染和根部定殖过程中受到抑制。我们还发现缺失[具体基因名称]会损害侵入栓的形成以及衍生的隔膜颈。破坏菌株是存活的，并且显示出正常的微菌核形成，而菌丝体生长和分生孢子产生减少，菌丝更短且分支更多。此外，与野生型[具体真菌名称]不同，[具体基因名称]突变体缺乏一个大的基部液泡，同时无法产生浓缩的脂滴。综上所述，[具体基因名称]通过[具体途径]调节液泡形成，这在分生孢子形成、菌丝体生长以及植物根部初始感染期间的穿透结构形成过程中是必需的。
