Faisal Lutfi Mohamed, Abdel-Moneim Abdel-Moneim Hafez, Alsharidah Ashwag Saleh, Mobark Mugahid A, Abdellatif Ahmed A H, Saleem Imran Y, Al Rugaie Osamah, Mohany Khalid M, Alsharidah Mansour
Department of Physiology, College of Medicine, Qassim University, Buraydah 51452, Saudi Arabia.
Department of Physiology, Faculty of Medicine, Nile College, Sheikh Zayed 7121, Sudan.
Molecules. 2021 Apr 17;26(8):2348. doi: 10.3390/molecules26082348.
The aim of the present study was to assess the short-term effects of Thymoquinone (TQ) on oxidative stress, glycaemic control, and renal functions in diabetic rats. DM was induced in groups II and III with a single dose of streptozotocin (STZ), while group I received no medication (control). The rats in groups I and II were then given distilled water, while the rats in group III were given TQ at a dose of 50 mg/kg body weight/day for 4 weeks. Lipid peroxidase, nitric oxide (NO), total antioxidant capacity (TAC), glycated haemoglobin (HbA1c), lipid profiles, and renal function were assessed. Moreover, the renal tissues were used for histopathological examination. STZ increased the levels of HbA1c, lipid peroxidase, NO, and creatinine in STZ-induced diabetic rats in comparison to control rats. TAC was lower in STZ-induced diabetic rats than in the control group. Furthermore, rats treated with TQ exhibited significantly lower levels of HbA1c, lipid peroxidase, and NO than did untreated diabetic rats. TAC was higher in diabetic rats treated with TQ than in untreated diabetic rats. The histopathological results showed that treatment with TQ greatly attenuated the effect of STZ-induced diabetic nephropathy. TQ effectively adjusts glycaemic control and reduces oxidative stress in STZ-induced diabetic rats without significant damaging effects on the renal function.
本研究的目的是评估百里醌（TQ）对糖尿病大鼠氧化应激、血糖控制和肾功能的短期影响。第二组和第三组用单剂量链脲佐菌素（STZ）诱导糖尿病，而第一组不接受药物治疗（对照组）。然后，第一组和第二组的大鼠给予蒸馏水，而第三组的大鼠给予剂量为50mg/kg体重/天的TQ，持续4周。评估脂质过氧化物酶、一氧化氮（NO）、总抗氧化能力（TAC）、糖化血红蛋白（HbA1c）、血脂谱和肾功能。此外，肾组织用于组织病理学检查。与对照大鼠相比，STZ增加了STZ诱导的糖尿病大鼠的HbA1c、脂质过氧化物酶、NO和肌酐水平。STZ诱导的糖尿病大鼠的TAC低于对照组。此外，用TQ治疗的大鼠的HbA1c、脂质过氧化物酶和NO水平显著低于未治疗的糖尿病大鼠。用TQ治疗的糖尿病大鼠的TAC高于未治疗的糖尿病大鼠。组织病理学结果表明，TQ治疗大大减轻了STZ诱导的糖尿病肾病的影响。TQ有效调节STZ诱导的糖尿病大鼠的血糖控制并降低氧化应激，而对肾功能无明显损害作用。
