Arumugam Paramasivam, Subramanian Raghunandhakumar, Priyadharsini Jayaseelan Vijayashree, Gopalswamy Jayaraman
Department of Genetics, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar campus, Taramani, Chennai-600113, India.
Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600025, India.
Chin J Nat Med. 2016 Dec;14(12):904-912. doi: 10.1016/S1875-5364(17)30015-8.
Thymoquinone (TQ), an active component derived from the medial plant Nigella sativa, has been used for medical purposes for more than 2 000 years. Recent studies have reported that TQ blocked angiogenesis in animal model and reduced migration, adhesion, and invasion of glioblastoma cells. We have recently shown that TQ could exhibit a potent cytotoxic effect and induce apoptosis in mouse neuroblastoma (Neuro-2a) cells. In the present study, TQ treatment markedly decreased the adhesion and migration of Neuro-2a cells. TQ down-regulated MMP-2 and MMP-9 protein expression and mRNA levels and their activities. Furthermore, TQ significantly down-regulated the protein expression of transcription factor NF-κB (p65) but not significantly altered the expression of N-Myc. Taken together, our data indicated that TQ's inhibitory effect on the migration of Neuro-2a cells was mediated through the suppression of MMP-2 and MMP-9 expression, suggesting that TQ treatment can be a promising therapeutic strategy for human malignant neuroblastoma.
百里醌（TQ）是从药用植物黑种草中提取的一种活性成分，两千多年来一直被用于医学用途。最近的研究报道，TQ在动物模型中可抑制血管生成，并减少胶质母细胞瘤细胞的迁移、黏附和侵袭。我们最近发现，TQ可对小鼠神经母细胞瘤（Neuro-2a）细胞产生强大的细胞毒性作用并诱导其凋亡。在本研究中，TQ处理显著降低了Neuro-2a细胞的黏附和迁移能力。TQ下调了基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）的蛋白表达、mRNA水平及其活性。此外，TQ显著下调了转录因子核因子κB（p65）的蛋白表达，但对N-Myc的表达没有显著影响。综上所述，我们的数据表明，TQ对Neuro-2a细胞迁移的抑制作用是通过抑制MMP-2和MMP-9的表达介导的，这表明TQ治疗可能是一种有前景的人类恶性神经母细胞瘤治疗策略。
