Girelli Chiara Roberta, Del Coco Laura, Angilè Federica, Scortichini Marco, Fanizzi Francesco Paolo
Department of Biological and Environmental Sciences and Technologies, University of Salento, Prov.le Lecce-Monteroni, 73100 Lecce, Italy.
Council for Research in Agriculture and Economics-Research Centre for Olive, Fruit and Citrus Crops, Via di Fioranello 52, I-00134 Roma, Italy.
Plants (Basel). 2021 Apr 15;10(4):772. doi: 10.3390/plants10040772.
subsp. is a bacterial phytopathogen associated with the "olive quick decline syndrome" (OQDS) causing severe economic losses to olive groves in Salento area (Apulia, Italy). In a previous work, we analyzed by H-NMR the metabolic pattern of naturally infected Ogliarola salentina and Cellina di Nardò susceptible cultivars untreated and treated with a zinc-copper citric acid biocomplex and we observed the treatment related variation of the disease biomarker quinic acid. In this study, we focused also on the Leccino cultivar, known to exhibit tolerance to the disease progression. The H-NMR-based metabolomic approach was applied with the aim to characterize the overall metabolism of tolerant Leccino in comparison with the susceptible cultivars Ogliarola salentina and Cellina di Nardò under periodic mid-term treatment. In particular, we studied the leaf extract molecular patterns of naturally infected trees untreated and treated with the biocomplex. The metabolic Leccino profiles were analyzed for the first time and compared with those exhibited by the susceptible Cellina di Nardò and Ogliarola salentina cultivars. The study highlighted a specificity in the metabolic response of the tolerant Leccino compared to susceptible cultivars. These differences provide useful information to describe the defensive mechanisms underlying the change of metabolites as a response to the infection, and the occurrence of different levels of disease, season and treatment effects for olive cultivars.
亚种是一种与“橄榄快速衰退综合征”（OQDS）相关的细菌性植物病原体，给萨伦托地区（意大利普利亚）的橄榄园造成了严重的经济损失。在之前的一项工作中，我们通过氢核磁共振（H-NMR）分析了未处理以及用锌-铜柠檬酸生物复合物处理的自然感染的奥利亚罗拉·萨伦蒂纳和纳尔多的切利纳易感品种的代谢模式，并且我们观察到了与疾病生物标志物奎尼酸相关的处理引起的变化。在本研究中，我们还聚焦于已知对疾病进展具有耐受性的莱基诺品种。应用基于H-NMR的代谢组学方法，旨在与易感品种奥利亚罗拉·萨伦蒂纳和纳尔多的切利纳相比，在中期定期处理下表征耐受性莱基诺的整体代谢。具体而言，我们研究了未处理以及用生物复合物处理的自然感染树木的叶片提取物分子模式。首次分析了莱基诺的代谢谱，并将其与易感品种纳尔多的切利纳和奥利亚罗拉·萨伦蒂纳所表现出的代谢谱进行比较。该研究突出了耐受性莱基诺与易感品种相比在代谢反应上的特异性。这些差异为描述作为对感染的反应代谢物变化背后的防御机制，以及橄榄品种不同水平的疾病、季节和处理效应的发生提供了有用信息。
