Department of Mechanical Engineering, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.
Degree Program of Automation and Precision Engineering, College of Engineering, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.
Sensors (Basel). 2021 Apr 15;21(8):2797. doi: 10.3390/s21082797.
This paper presents a compact spectral detection system for common fluorescent and colorimetric assays. This system includes a gradient grating period guided-mode resonance (GGP-GMR) filter and charge-coupled device. In its current form, the GGP-GMR filter, which has a size of less than 2.5 mm, can achieve a spectral detection range of 500-700 nm. Through the direct measurement of the fluorescence emission, the proposed system was demonstrated to detect both the peak wavelength and its corresponding intensity. One fluorescent assay (albumin) and two colorimetric assays (albumin and creatinine) were performed to demonstrate the practical application of the proposed system for quantifying common liquid assays. The results of our system exhibited suitable agreement with those of a commercial spectrometer in terms of the assay sensitivity and limit of detection (LOD). With the proposed system, the fluorescent albumin, colorimetric albumin, and colorimetric creatinine assays achieved LODs of 40.99 and 398 and 25.49 mg/L, respectively. For a wide selection of biomolecules in point-of-care applications, the spectral detection range achieved by the GGP-GMR filter can be further extended and the simple and compact optical path configuration can be integrated with a lab-on-a-chip system.
本文提出了一种用于常见荧光和比色分析的紧凑型光谱检测系统。该系统包括梯度光栅周期导模共振（GGP-GMR）滤波器和电荷耦合器件。在目前的形式下，尺寸小于 2.5 毫米的 GGP-GMR 滤波器可以实现 500-700nm 的光谱检测范围。通过对荧光发射的直接测量，该系统被证明可以同时检测峰值波长及其相应的强度。进行了一项荧光分析（白蛋白）和两项比色分析（白蛋白和肌酸酐），以证明所提出的系统在定量常见液体分析方面的实际应用。我们的系统的结果在分析灵敏度和检测限（LOD）方面与商用分光光度计具有良好的一致性。使用所提出的系统，荧光白蛋白、比色白蛋白和比色肌酸酐分析的 LOD 分别达到了 40.99、398 和 25.49mg/L。对于在即时护理应用中广泛选择的生物分子，可以进一步扩展 GGP-GMR 滤波器的光谱检测范围，并将简单紧凑的光路配置集成到芯片实验室系统中。
