Katsarou Eleni I, Katsafadou Angeliki I, Karakasidis Theodoros, Chatzopoulos Dimitris C, Vasileiou Natalia G C, Lianou Daphne T, Mavrogianni Vasia S, Petinaki Efthymia, Fthenakis George C
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece.
Faculty of Public and Integrated (One) Health, University of Thessaly, 43100 Karditsa, Greece.
Microorganisms. 2021 Apr 15;9(4):852. doi: 10.3390/microorganisms9040852.
The growth of two isolates (one biofilm-forming and one not) on teatcups for cattle (made of rubber) or sheep (made of silicone) were assessed in nine multiplicates for 24 h post-smearing on the teatcup surface. Staphylococci were smeared on an area of 0.0003142 m on the material and their growth and expansion further on were monitored for 24 h. There were no differences in the frequency of recoveries between the two isolates ( > 0.82 for all comparisons). There were more recoveries from sheep teatcups than from cattle teatcups: 1280/1728 (74.1%) versus 942/1728 (54.5%), for both isolates ( < 0.0001). Significance was observed only 6 h to 15 h after smearing ( < 0.0001 for all comparisons). The median speed of linear dissemination of the isolates was 0.00000021 m s on cattle teatcups and 0.00000033 m s on sheep teatcups ( < 0.0001). The increased growth and faster expansion of staphylococci on silicone teatcups raise important points from a clinical viewpoint. The model could be used in the testing of staphylococcal growth in the material of milking parlours in various conditions.
在奶牛（由橡胶制成）或绵羊（由硅胶制成）的奶杯表面涂抹两种葡萄球菌分离株（一种形成生物膜，一种不形成生物膜）后，以九个重复样本评估其在24小时内的生长情况。将葡萄球菌涂抹在材料上面积为0.0003142平方米的区域，并监测其在接下来24小时内的生长和扩散情况。两种分离株的回收率频率没有差异（所有比较均>0.82）。从绵羊奶杯上回收的细菌比从奶牛奶杯上回收的更多：两种分离株分别为1280/1728（74.1%）和942/1728（54.5%）（<0.0001）。仅在涂抹后6小时至15小时观察到显著差异（所有比较均<0.0001）。分离株在奶牛奶杯上的线性传播中位速度为0.00000021米/秒，在绵羊奶杯上为0.00000033米/秒（<0.0001）。从临床角度来看，葡萄球菌在硅胶奶杯上生长增加且扩散更快这一情况提出了重要问题。该模型可用于测试在各种条件下挤奶厅材料中葡萄球菌的生长情况。
