Department of Orthopaedic Surgery, University Hospital Tübingen, 72072 Tübingen, Germany.
Department of Urology, University Hospital Tübingen, Waldhörnlestrasse 22, 72072 Tübingen, Germany.
Int J Mol Sci. 2021 Apr 12;22(8):3958. doi: 10.3390/ijms22083958.
Previously, we developed a novel, needle-free waterjet (WJ) technology capable of injecting viable cells by visual guided cystoscopy in the urethral sphincter. In the present study, we aimed to investigate the effect of WJ technology on cell viability, surface markers, differentiation and attachment capabilities, and biomechanical features. Porcine adipose tissue-derived stromal cells (pADSCs) were isolated, expanded, and injected by WJ technology. Cell attachment assays were employed to investigate cell-matrix interactions. Cell surface molecules were analyzed by flow cytometry. Cells injected by Williams Needle (WN), normal cannula, or not injected cells served as controls. Biomechanical properties were assessed by atomic force microscopy (AFM). pADSCs injected by the WJ were viable (85.9%), proliferated well, and maintained their in vitro adipogenic and osteogenic differentiation capacities. The attachment of pADSCs was not affected by WJ injection and no major changes were noted for cell surface markers. AFM measurements yielded a significant reduction of cellular stiffness after WJ injections ( < 0.001). WJ cell delivery satisfies several key considerations required in a clinical context, including the fast, simple, and reproducible delivery of viable cells. However, the optimization of the WJ device may be necessary to further reduce the effects on the biomechanical properties of cells.
此前，我们开发了一种新型的无针水射流（WJ）技术，能够通过尿道括约肌的视觉引导膀胱镜将活细胞注入。在本研究中，我们旨在研究 WJ 技术对细胞活力、表面标志物、分化和附着能力以及生物力学特性的影响。从猪脂肪组织中分离、扩增并通过 WJ 技术注射基质细胞（pADSCs）。通过细胞附着实验研究细胞-基质相互作用。通过流式细胞术分析细胞表面分子。将通过 Williams 针（WN）、普通套管或未注射细胞注射的细胞作为对照。通过原子力显微镜（AFM）评估生物力学特性。通过 WJ 注射的 pADSCs 具有活力（85.9%），增殖良好，并保持其体外成脂和成骨分化能力。WJ 注射不会影响 pADSCs 的附着，并且细胞表面标志物没有明显变化。AFM 测量结果表明，WJ 注射后细胞刚度显著降低（<0.001）。WJ 细胞输送满足临床环境所需的几个关键考虑因素，包括快速、简单和可重复的活细胞输送。然而，可能需要优化 WJ 装置以进一步降低对细胞生物力学特性的影响。
