Chang Cheng-Yi, Pan Ping-Ho, Li Jian-Ri, Ou Yen-Chuan, Liao Su-Lan, Chen Wen-Ying, Kuan Yu-Hsiang, Chen Chun-Jung
Department of Surgery, Feng Yuan Hospital, Taichung City 420, Taiwan.
Department of Veterinary Medicine, National Chung Hsing University, Taichung City 402, Taiwan.
Antioxidants (Basel). 2021 Apr 19;10(4):623. doi: 10.3390/antiox10040623.
In stroke patients, the development of acute kidney injury (AKI) is closely linked with worse outcomes and increased mortality. In this study, the interplay between post-stroke and AKI and treatment options was investigated in a rodent model of hemorrhagic stroke. Intrastriatal collagenase injection for 24 h caused neurological deficits, hematoma formation, brain edema, apoptosis, blood-brain barrier disruption, oxidative stress, and neuroinflammation in Sprague Dawley rats. Elevation of serum blood urea nitrogen, serum creatinine, urine cytokine-induced neutrophil chemoattractant-1, and urine Malondialdehyde, as well as moderate histological abnormality in the kidney near the glomerulus, indicated evidence of kidney dysfunction. The accumulation of podocalyxin DNA in urine further suggested a detachment of podocytes and structural deterioration of the glomerulus. Circulating levels of stress hormones, such as epinephrine, norepinephrine, corticosterone, and angiotensin II were elevated in rats with intracerebral hemorrhage. Osmotic agent glycerol held promising effects in alleviating post-stroke brain injury and kidney dysfunction. Although the detailed protective mechanisms of glycerol have yet to be determined, the intrastriatal collagenase injection hemorrhagic stroke model in rats allowed us to demonstrate the functional and structural integrity of glomerulus are targets that are vulnerable to post-stroke injury and stress hormones could be surrogates of remote communications.
在中风患者中，急性肾损伤（AKI）的发生与更差的预后和更高的死亡率密切相关。在本研究中，在出血性中风的啮齿动物模型中研究了中风后与AKI之间的相互作用以及治疗方案。对Sprague Dawley大鼠纹状体内注射胶原酶24小时会导致神经功能缺损、血肿形成、脑水肿、细胞凋亡、血脑屏障破坏、氧化应激和神经炎症。血清血尿素氮、血清肌酐、尿细胞因子诱导的中性粒细胞趋化因子-1和尿丙二醛升高，以及肾小球附近肾脏出现中度组织学异常，均表明存在肾功能障碍的证据。尿中足细胞标记蛋白DNA的积累进一步提示足细胞脱离和肾小球结构破坏。脑出血大鼠体内肾上腺素、去甲肾上腺素、皮质酮和血管紧张素II等应激激素的循环水平升高。渗透剂甘油在减轻中风后脑损伤和肾功能障碍方面具有良好效果。尽管甘油的详细保护机制尚未确定，但大鼠纹状体内注射胶原酶出血性中风模型使我们能够证明肾小球的功能和结构完整性是易受中风后损伤的靶点，应激激素可能是远程通讯的替代物。
