Murakami M, Ushio Y, Morino Y, Ohta T, Matsukado Y
Department of Neurosurgery, Kumamoto University Medical School, Japan.
J Clin Invest. 1988 Jul;82(1):177-88. doi: 10.1172/JCI113568.
Immunocytochemical analyses revealed the presence and distribution of apolipoprotein E (apo E) in normal human brain tissue as well as in 77 human intracranial neoplasms. In normal brain tissues, the perikarya of astrocytes exhibited a strong positive reaction, whereas the Bergmann glia were stained to a moderate degree. However, no immunoreactivity was observed with neurons, oligodendrocytes, ependymal cells, and choroidal epithelium. Among the intracranial neoplasms, oligodendroglioma, choroid plexus papilloma, hemangioblastoma, primary malignant lymphoma, neurinoma, meningioma, pituitary adenoma, and craniopharyngioma were all negative. Immunoreactivity in the peripheral neuroblastoma was nil. However, the perikarya of astrocytomas and glioblastomas showed a positive reaction. Analyses on the degree of anaplasia and the amount of apo-E as an intensity of immunostaining showed a negative correlation. The astrocytic elements were stained in mixed oligoastrocytomas and medulloblastomas with glial differentiation. A few cases of ependymomas showed weak perikaryal immunostaining. Western blot analyses with anti-apo E antibody of a freshly prepared surgical specimen with astrocytomas revealed a single band with a molecular weight of approximately 37,000. The well differentiated cultured human astrocytoma cells secreted apo E into the medium. These lines of evidence suggest that apo E may serve as a potential marker specific for astrocytomas and glioblastomas, as well as an indicator of astrocytic tumor cell differentiation. The apo E localization in human brain tumors could be clinically relevant and diagnostically useful.
免疫细胞化学分析揭示了载脂蛋白E（apo E）在正常人类脑组织以及77例人类颅内肿瘤中的存在和分布情况。在正常脑组织中，星形胶质细胞的胞体呈现出强烈的阳性反应，而伯格曼胶质细胞则呈中度染色。然而，在神经元、少突胶质细胞、室管膜细胞和脉络丛上皮细胞中未观察到免疫反应性。在颅内肿瘤中，少突胶质细胞瘤、脉络丛乳头状瘤、成血管细胞瘤、原发性恶性淋巴瘤、神经鞘瘤、脑膜瘤、垂体腺瘤和颅咽管瘤均为阴性。外周神经母细胞瘤的免疫反应性为零。然而，星形细胞瘤和胶质母细胞瘤的胞体呈现出阳性反应。对间变程度和作为免疫染色强度的apo - E量的分析显示呈负相关。在混合性少突星形细胞瘤和具有胶质分化的髓母细胞瘤中，星形细胞成分被染色。少数室管膜瘤病例显示出微弱的胞体免疫染色。用抗apo E抗体对新鲜制备的星形细胞瘤手术标本进行蛋白质印迹分析，显示出一条分子量约为37,000的条带。分化良好的培养人星形细胞瘤细胞将apo E分泌到培养基中。这些证据表明，apo E可能作为星形细胞瘤和胶质母细胞瘤的潜在特异性标志物，以及星形细胞瘤细胞分化的指标。apo E在人脑肿瘤中的定位可能具有临床相关性和诊断价值。
