Oster Michael, Reyer Henry, Gerlinger Christian, Trakooljul Nares, Siengdee Puntita, Keiler Jonas, Ponsuksili Siriluck, Wolf Petra, Wimmers Klaus
Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany.
Department of Anatomy, Rostock University Medical Center, 18057 Rostock, Germany.
Biomedicines. 2021 Apr 22;9(5):454. doi: 10.3390/biomedicines9050454.
Knowledge of gene expression profiles reflecting functional features and specific responsiveness of parathyroid glands (PTGs) contributes to understanding mineral homeostasis and parathyroid function in healthy and diseased conditions. The study aims to reveal effector molecules driving the maintenance of phosphorus (P) homeostasis and parathyroid hormone (PTH) responsiveness to variable P supply throughout fetal and postnatal life. In this study, a long-term dietary intervention was performed by keeping pig offspring on distinct mineral P levels throughout fetal and postnatal life. Respective adaptation processes of P homeostasis were assessed in mRNA profiles of PTGs and serum minerals. RNA sequencing data and resulting molecular pathways of PTGs showed that the PTH abundance is very strictly controlled via e.g., , , , PLC and PKA signaling to regulate PTH expression, stability, and secretion. Additionally, the observed dietary effects on collagen expression indicate shifts in the ratio between connective tissue and parenchyma, thereby affecting cell-cell contacts as another line of PTH regulation. Taken together, the mRNA profiles of porcine PTGs reflect physiological responses in-vivo following variable dietary P supplies during fetal and postnatal life. The results serve to evaluate a long-term nutrition strategy with implications for improving the mineral balance in individuals with pathological disorders.
了解反映甲状旁腺（PTG）功能特征和特定反应性的基因表达谱，有助于理解健康和疾病状态下的矿物质稳态及甲状旁腺功能。本研究旨在揭示在胎儿期和出生后整个生命过程中驱动磷（P）稳态维持以及甲状旁腺激素（PTH）对不同磷供应产生反应的效应分子。在本研究中，通过在胎儿期和出生后整个生命过程中使猪后代维持不同的矿物质磷水平，进行了长期饮食干预。在PTG的mRNA谱和血清矿物质中评估了磷稳态的相应适应过程。PTG的RNA测序数据及由此产生的分子途径表明，PTH丰度通过例如PLC和PKA信号传导等受到非常严格的控制，以调节PTH的表达、稳定性和分泌。此外，观察到的饮食对胶原蛋白表达的影响表明结缔组织与实质之间的比例发生了变化，从而影响细胞间接触，这是PTH调节的另一条途径。综上所述，猪PTG的mRNA谱反映了胎儿期和出生后整个生命过程中不同饮食磷供应后体内的生理反应。这些结果有助于评估一项长期营养策略，对改善患有病理疾病个体的矿物质平衡具有重要意义。
