Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
Department of Cattle Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
Genes (Basel). 2022 Aug 9;13(8):1419. doi: 10.3390/genes13081419.
The goal of this study was to evaluate the effects of mutations in the gene associated with pig productive traits to enrich the genetic marker pool for further selection and to support the studies on in the context of the fat deposition (FD) process. The variant calling and χ analyses of liver RNA-seq data were used to indicate genetic markers. mutations were genotyped in the Złotnicka White (n = 72), Polish Large White (n = 208), Duroc (n = 72), Polish Landrace (PL) (n = 292), and Puławska (n = 178) pig breeds. An association study was performed using a general linear model (GLM) implemented in SAS software. More than 50 crucial mutations were identified in the gene. The association study showed a significant effect of the on intramuscular fat (IMF), loin eye area, backfat thickness at the lumbar, ham mass ( = 0.0374), meat percentage ( = 0.0205), and loin fat ( = 0.0003). Alternate homozygotes and heterozygotes were found in the PL and Duroc, confirming the selective potential for these populations. Our study supports the theory that liver is involved in the FD process. Moreover, since fat is the major determinant of flavor development in meat, the rs340465447_A allele can be used as a target in pig selection focused on elevated fat levels.
本研究旨在评估与猪生产性状相关基因中的突变对遗传标记的影响，以进一步选择和支持脂肪沉积（FD）过程中基因的研究。使用肝 RNA-seq 数据的变体调用和 χ 分析来指示遗传标记。在 Złotnicka White（n = 72）、波兰大白猪（n = 208）、杜洛克（n = 72）、波兰长白猪（PL）（n = 292）和 Puławska（n = 178）猪品种中对突变进行了基因分型。使用 SAS 软件中的一般线性模型（GLM）进行关联研究。在基因中鉴定出超过 50 个关键突变。关联研究表明基因对肌内脂肪（IMF）、腰眼面积、腰椎背膘厚、火腿质量（= 0.0374）、肉百分比（= 0.0205）和腰脂肪（= 0.0003）有显著影响。在 PL 和杜洛克中发现了交替纯合子和杂合子，证实了这些群体的选择潜力。我们的研究支持了肝脏基因参与 FD 过程的理论。此外，由于脂肪是肉中风味发展的主要决定因素，因此 rs340465447_A 等位基因可作为猪选择的目标，重点提高脂肪水平。
