Paz Sergio Montserrat-de la, Martinez-Lopez Alicia, Villanueva-Lazo Alvaro, Pedroche Justo, Millan Francisco, Millan-Linares Maria C
Department of Medical Biochemistry, Molecular Biology and Immunology, School of Medicine, Universidad de Sevilla, Av. Sanchez Pizjuan s/n, 41009 Seville, Spain.
Plant Protein Group, Department of Food and Health, Instituto de la Grasa, CSIC, Ctra. Utrera Km 1, 41013 Seville, Spain.
Antioxidants (Basel). 2021 Apr 22;10(5):645. doi: 10.3390/antiox10050645.
Kiwicha () is considered one of the few multipurpose pseudocereals for its potential use not only as a source of nutrients and fiber but also for its bioactive compounds. In recent years, antioxidant peptides are commonly used as functional ingredient of food. Herein, a kiwicha protein isolate (KPI), obtained from kiwicha defatted flour (KDF), was hydrolyzed by Bioprotease LA 660, a food-grade endoprotease, under specific conditions. The resulting kiwicha protein hydrolysates (KPHs) were chemically characterized and their digestibility and antioxidant capacity were evaluated by in vitro cell-free experiments owing to their measure of capacity to sequester DPPH free radical and reducing power. KPHs showed higher digestibility and antioxidant capacity than intact proteins into KPI. Therefore, the results shown in this study indicate that KPHs could serve as an adequate source of antioxidant peptides, representing an effective alternative to the generation of functional food.
藜麦（）因其不仅有可能作为营养和纤维来源，还因其生物活性化合物而被认为是少数几种多用途的伪谷物之一。近年来，抗氧化肽通常用作食品的功能成分。在此，从藜麦脱脂面粉（KDF）中获得的藜麦分离蛋白（KPI）在特定条件下被食品级内切蛋白酶Bioprotease LA 660水解。通过体外无细胞实验对所得的藜麦蛋白水解物（KPHs）进行化学表征，并评估其消化率和抗氧化能力，这是由于它们具有螯合DPPH自由基的能力和还原能力。与KPI中的完整蛋白质相比，KPHs表现出更高的消化率和抗氧化能力。因此，本研究结果表明，KPHs可以作为抗氧化肽的合适来源，代表了生产功能性食品的有效替代方法。
