Balmasova Irina P, Olekhnovich Evgenii I, Klimina Ksenia M, Korenkova Anna A, Vakhitova Maria T, Babaev Elmar A, Ovchinnikova Leyla A, Lomakin Yakov A, Smirnov Ivan V, Tsarev Victor N, Mkrtumyan Ashot M, Belogurov Alexey A, Gabibov Alexander G, Ilina Elena N, Arutyunov Sergey D
Ministry of Healthcare of the Russian Federation, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 127473 Moscow, Russia.
Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Medical and Biological Agency of Russia, 119435 Moscow, Russia.
Pathogens. 2021 Apr 22;10(5):504. doi: 10.3390/pathogens10050504.
Since periodontitis and type 2 diabetes mellitus are complex diseases, a thorough understanding of their pathogenesis requires knowing the relationship of these pathologies with other disorders and environmental factors. In this study, the representability of the subgingival periodontal microbiome of 46 subjects was studied by 16S rRNA gene sequencing and shotgun sequencing of pooled samples. We examined 15 patients with chronic periodontitis (CP), 15 patients with chronic periodontitis associated with type 2 diabetes mellitus (CPT2DM), and 16 healthy subjects (Control). The severity of generalized chronic periodontitis in both periodontitis groups of patients (CP and CPT2DM) was moderate (stage II). The male to female ratios were approximately equal in each group (22 males and 24 females); the average age of the subjects was 53.9 ± 7.3 and 54.3 ± 7.2 years, respectively. The presence of overweight patients (Body Mass Index (BMI) 30-34.9 kg/m) and patients with class 1-2 obesity (BMI 35-45.9 kg/m) was significantly higher in the CPT2DM group than in patients having only chronic periodontitis or in the Control group. However, there was no statistically significant difference in all clinical indices between the CP and CPT2DM groups. An analysis of the metagenomic data revealed that the alpha diversity in the CPT2DM group was increased compared to that in the CP and Control groups. The microbiome biomarkers associated with experimental groups were evaluated. In both groups of patients with periodontitis, the relative abundance of was increased compared to that in the Control group. The CPT2DM group was characterized by a lower relative abundance of / and a higher abundance of compared to those in the CP and Control groups. Furthermore, the CP and CPT2DM groups differed in terms of the relative abundance of (which was decreased in the CPT2DM group compared to CP) and (which was increased in the CPT2DM group compared to CP). In addition, differences in bacterial content were identified by a combination of shotgun sequencing of pooled samples and genome-resolved metagenomics. The results indicate that there are subgingival microbiome-specific features in patients with chronic periodontitis associated with type 2 diabetes mellitus.
由于牙周炎和2型糖尿病都是复杂的疾病，要全面了解它们的发病机制，就需要知晓这些病理状况与其他疾病及环境因素之间的关系。在本研究中，通过对混合样本进行16S rRNA基因测序和鸟枪法测序，研究了46名受试者龈下牙周微生物群的代表性。我们检查了15名慢性牙周炎（CP）患者、15名伴有2型糖尿病的慢性牙周炎（CPT2DM）患者以及16名健康受试者（对照组）。两组牙周炎患者（CP和CPT2DM）中广泛性慢性牙周炎的严重程度均为中度（II期）。每组的男女比例大致相等（男性22名，女性24名）；受试者的平均年龄分别为53.9±7.3岁和54.3±7.2岁。CPT2DM组中超重患者（体重指数（BMI）30 - 34.9 kg/m²）和1 - 2级肥胖患者（BMI 35 - 45.9 kg/m²）的比例显著高于仅患有慢性牙周炎的患者或对照组。然而，CP组和CPT2DM组之间的所有临床指标均无统计学显著差异。宏基因组数据分析显示，CPT2DM组的α多样性相较于CP组和对照组有所增加。对与实验组相关的微生物群生物标志物进行了评估。在两组牙周炎患者中，[此处原文缺失具体内容]的相对丰度相较于对照组均有所增加。与CP组和对照组相比，CPT2DM组的特征在于[此处原文缺失具体内容]的相对丰度较低，而[此处原文缺失具体内容]的丰度较高。此外，CP组和CPT2DM组在[此处原文缺失具体内容]（与CP组相比，CPT2DM组中该指标降低）和[此处原文缺失具体内容]（与CP组相比，但CPT2DM组中该指标升高）的相对丰度方面存在差异。此外，通过对混合样本进行鸟枪法测序和基因组解析宏基因组学相结合的方法，确定了细菌含量上的差异。结果表明，伴有2型糖尿病的慢性牙周炎患者存在龈下微生物群特异性特征。
