de Eugenio Laura Isabel, Peces-Pérez Rosa, Linde Dolores, Prieto Alicia, Barriuso Jorge, Ruiz-Dueñas Francisco Javier, Martínez María Jesús
Centro de Investigaciones Biológicas Margarita Salas (CIB), Consejo Superior de Investigaciones Científicas (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.
J Fungi (Basel). 2021 Apr 22;7(5):325. doi: 10.3390/jof7050325.
A dye-decolorizing peroxidase (DyP) from was cloned and heterologously expressed as inclusion bodies in . The protein was purified in one chromatographic step after its in vitro activation. It was active on ABTS, 2,6-dimethoxyphenol (DMP), and anthraquinoid and azo dyes as reported for other fungal DyPs, but it was also able to oxidize Mn (as manganese peroxidases and versatile peroxidases) and veratryl alcohol (VA) (as lignin peroxidases and versatile peroxidases). This corroborated that DyPs are the only enzymes able to oxidize high redox potential dyes, VA and Mn. Phylogenetic analysis grouped this enzyme with other type D-DyPs from basidiomycetes. In addition to its interest for dye decolorization, the results of the transformation of softwood and hardwood lignosulfonates suggest a putative biological role of this enzyme in the degradation of phenolic lignin.
从[来源]克隆了一种染料脱色过氧化物酶（DyP），并在[表达系统]中作为包涵体进行异源表达。该蛋白在体外激活后通过一步色谱法进行纯化。如其他真菌DyP报道的那样，它对ABTS、2,6 - 二甲氧基苯酚（DMP）以及蒽醌和偶氮染料具有活性，但它也能够氧化锰（如锰过氧化物酶和多功能过氧化物酶）和藜芦醇（VA）（如木质素过氧化物酶和多功能过氧化物酶）。这证实了[来源]DyP是唯一能够氧化高氧化还原电位染料、VA和锰的酶。系统发育分析将该酶与来自担子菌的其他D型DyP归为一类。除了其在染料脱色方面的研究价值外，软木和硬木木质素磺酸盐转化的结果表明该酶在酚类木质素降解中可能具有生物学作用。
