Department of Biophysics of Environmental Pollution, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska Str. 141/143, 90-236 Lodz, Poland.
Int J Mol Sci. 2021 Apr 23;22(9):4406. doi: 10.3390/ijms22094406.
With the ongoing commercialization, human exposure to plastic nanoparticles will dramatically increase, and evaluation of their potential toxicity is essential. There is an ongoing discussion on the human health effects induced by plastic particles. For this reason, in our work, we assessed the effect of polystyrene nanoparticles (PS-NPs) of various diameters (29, 44 and 72 nm) on selected parameters of oxidative stress and the viability of human peripheral blood mononuclear cells (PBMCs) in the in vitro system. Cells were incubated with PS-NPs for 24 h in the concentration range of 0.001 to 100 µg/mL and then labeled: formation of reactive oxygen species (ROS) (including hydroxyl radical), protein and lipid oxidation and cell viability. We showed that PS-NPs disturbed the redox balance in PBMCs. They increased ROS levels and induced lipid and protein oxidation, and, finally, the tested nanoparticles induced a decrease in PBMCs viability. The earliest changes in the PBMCs were observed in cells incubated with the smallest PS-NPs, at a concentration of 0.01 μg/mL. A comparison of the action of the studied nanoparticles showed that PS-NPs (29 nm) exhibited a stronger oxidative potential in PBMCs. We concluded that the toxicity and oxidative properties of the PS-NPs examined depended to significant degree on their diameter.
随着商业化的不断发展，人类接触塑料纳米颗粒的情况将大幅增加，因此评估其潜在毒性至关重要。目前正在讨论塑料颗粒对人类健康的影响。基于此，我们在体外系统中评估了不同直径（29、44 和 72nm）的聚苯乙烯纳米颗粒（PS-NPs）对氧化应激相关参数和人外周血单个核细胞（PBMCs）活力的影响。细胞在 0.001 至 100µg/mL 的浓度范围内与 PS-NPs 孵育 24 小时，然后用标记法检测：活性氧（ROS）（包括羟自由基）、蛋白质和脂质氧化以及细胞活力的形成。结果表明 PS-NPs 破坏了 PBMCs 中的氧化还原平衡。它们增加了 ROS 水平，诱导了脂质和蛋白质氧化，最终导致测试的纳米颗粒降低了 PBMCs 的活力。在浓度为 0.01µg/mL 时，最小的 PS-NPs 作用于细胞，最早引起 PBMCs 的变化。对研究中纳米颗粒的作用进行比较后发现，PS-NPs（29nm）在 PBMCs 中表现出更强的氧化潜能。我们得出结论，所研究的 PS-NPs 的毒性和氧化特性在很大程度上取决于其直径。
