Rubio Laura, Barguilla Irene, Domenech Josefa, Marcos Ricard, Hernández Alba
Nanobiology Laboratory, Department of Natural and Exact Sciences, Pontificia Universidad Católica Madre y Maestra, PUCMM, Santiago de los Caballeros, Dominican Republic.
Department of Genetics and Microbiology, Faculty of Biosciences, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain.
J Hazard Mater. 2020 Nov 5;398:122900. doi: 10.1016/j.jhazmat.2020.122900. Epub 2020 May 15.
In recent years the terms "micro-/nanoplastics" (MNPLs) have caught special attention due to the increasing levels by which humans are exposed. Among MNPLs, polystyrene nanoparticles (PSNPs) are one of the most represented MNPLs in the environment. These tiny particles may enter into the human body, translocate through human barriers, interacting with blood and lymphatic immune cells, and reaching secondary organs. By using three different human leukocytic cell lines: Raji-B (B-lymphocytes), TK6 (lymphoblasts) and THP-1 (monocytes), we pursued to determine the effects of these PSNPs on the immune cell population. With this aim, the three cell lines were exposed to spherical PSNPs of about 50 nm of diameter and cytotoxicity, cellular uptake, reactive oxygen species (ROS) production, and genotoxicity were assessed at different time-points. Results show differences in all the measured endpoints, among the selected cell lines. Thus, whilst the monocytic THP-1 cells showed the highest particle internalization, no adverse effects were observed in such cells. On the other side, although Raji-B and TK6 showed lesser PSNPs uptake, mild toxicity, ROS production and genotoxicity were detected. These results highlight the importance of the cell line selection when the biological effects of PSNPs are evaluated.
近年来，“微塑料/纳米塑料”（MNPLs）一词因人类接触水平的不断提高而备受关注。在MNPLs中，聚苯乙烯纳米颗粒（PSNPs）是环境中最具代表性的MNPLs之一。这些微小颗粒可能进入人体，穿过人体屏障，与血液和淋巴免疫细胞相互作用，并到达次级器官。通过使用三种不同的人类白细胞系：Raji-B（B淋巴细胞）、TK6（淋巴母细胞）和THP-1（单核细胞），我们试图确定这些PSNPs对免疫细胞群体的影响。为此，将这三种细胞系暴露于直径约50nm的球形PSNPs中，并在不同时间点评估细胞毒性、细胞摄取、活性氧（ROS）产生和遗传毒性。结果显示，在所选择的细胞系中，所有测量终点均存在差异。因此，虽然单核细胞THP-1细胞表现出最高的颗粒内化，但在这些细胞中未观察到不良反应。另一方面，尽管Raji-B和TK6显示出较少的PSNPs摄取，但检测到了轻度毒性、ROS产生和遗传毒性。这些结果突出了在评估PSNPs的生物学效应时细胞系选择的重要性。
