Zhu Xiaohan, Arfaoui Arbia, Sayari Mohammad, Adam Lorne R, Daayf Fouad
Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada.
Pathogens. 2021 Apr 23;10(5):510. doi: 10.3390/pathogens10050510.
Quantitative real-time PCR was used to monitor the expression of 15 's genes, putatively involved in pathogenicity, highly (HAV) and weakly aggressive (WAV) isolates after either (i) elicitation with potato leaf, stem, or root extracts, or (ii) inoculation of potato detached petioles. These genes, i.e., coding for Ras-GAP-like protein, serine/threonine protein kinase, Ubiquitin-conjugating enzyme variant-MMS2, NADH-ubiquinone oxidoreductase, Thioredoxin, Pyruvate dehydrogenase E1 VdPDHB, myo-inositol 2-dehydrogenase, and HAD-superfamily hydrolase, showed differential upregulation in the HAV versus WAV isolate in response to plant extracts or after inoculation of potato leaf petioles. This suggests their potential involvement in the observed differential aggressiveness between isolates. However, other genes like glucan endo-1,3-alpha-glucosidase and nuc-1 negative regulatory protein showed higher activity in the WAV than in the HAV in response to potato extracts and/or during infection. This, in contrast, may suggest a role in their lower aggressiveness. These findings, along with future functional analysis of selected genes, will contribute to improving our understanding of 's pathogenesis. For example, expression of negatively regulates phosphorus-acquisition enzymes, which may indicate a lower phosphorus acquisition activity in the WAV. Therefore, integrating the knowledge about the activity of both genes enhancing pathogenicity and those restraining it will provide a guild line for further functional characterization of the most critical genes, thus driving new ideas towards better Verticillium wilt management.
采用定量实时聚合酶链反应（Quantitative real-time PCR）监测15个假定参与致病性的基因在高致病性（HAV）和低致病性（WAV）分离株中的表达情况。这些分离株在以下两种情况下进行监测：（i）用马铃薯叶、茎或根提取物诱导后；（ii）接种马铃薯离体叶柄后。这些基因，即编码Ras-GAP样蛋白、丝氨酸/苏氨酸蛋白激酶、泛素结合酶变体-MMS2、NADH-泛醌氧化还原酶、硫氧还蛋白、丙酮酸脱氢酶E1 VdPDHB、肌醇2-脱氢酶和HAD超家族水解酶的基因，在HAV和WAV分离株中，对植物提取物或接种马铃薯叶柄后的反应表现出不同程度的上调。这表明它们可能参与了分离株间观察到的致病性差异。然而，其他基因，如葡聚糖内切-1,3-α-葡糖苷酶和nuc-1负调控蛋白，在对马铃薯提取物反应时和/或感染期间，在WAV中的活性高于HAV。相比之下，这可能表明它们在较低致病性中发挥作用。这些发现，连同对选定基因的未来功能分析，将有助于增进我们对[病原菌名称未给出]发病机制的理解。例如，[基因名称未给出]的表达负调控磷获取酶，这可能表明WAV中磷获取活性较低。因此，整合关于增强致病性的基因和抑制致病性的基因活性的知识，将为进一步对最关键基因进行功能表征提供指导方针，从而推动新的思路以更好地管理黄萎病。
