Mace M E, Stipanovic R D, Bell A A
USDA, ARS, Southern Crops Research Laboratory, Cotton Pathology Research Unit, College Station, TX, 77841, USA.
New Phytol. 1989 Feb;111(2):229-232. doi: 10.1111/j.1469-8137.1989.tb00687.x.
The terpenoid phytoalexin desoxyhemigossypol (dHG) was detected histochemically in the stem xyiem of Verticillium dahliae Kleb-infected, wilt-resistant Seabrook Sea Island cotton as a green product on V. dahliae mycelium within vessel lumens and in specialized, often solitary, paravascular parenchyma cells. The SbCl -HClO histochemical reagent yielded a green-coloured Sb-dHG product specific for dHG when used as a spray on chromatograms of extracts from Verticillium-infected stele tissue. Both dHG and related terpenoid aldehyde derivatives occurred together in parenchyma cells and on V. dahliae mycelium. The presence of dHG on Verticillium mycelium reinforces previous studies that identified dHG as the most toxic and possibly most important phytoalexin in the resistance of cotton to V. dahliae.
在感染大丽轮枝菌（Verticillium dahliae Kleb）的抗枯萎海岛棉品种Seabrook的茎木质部中，通过组织化学方法检测到萜类植物抗毒素去氧半棉酚（dHG），它在导管腔内的大丽轮枝菌菌丝体上以及在特化的、通常为单个的维管束旁薄壁细胞中呈现为绿色产物。当将SbCl -HClO组织化学试剂喷洒在感染大丽轮枝菌的中柱组织提取物的色谱图上时，会产生一种对dHG特异的绿色Sb-dHG产物。dHG和相关的萜类醛衍生物同时存在于薄壁细胞和大丽轮枝菌菌丝体上。dHG在大丽轮枝菌菌丝体上的存在进一步证实了先前的研究，这些研究将dHG鉴定为棉花对大丽轮枝菌抗性中毒性最强且可能最重要的植物抗毒素。
