Díaz-Ríos Catalina, Hernández Marta, Abad David, Álvarez-Montes Laura, Varsaki Athanasia, Iturbe David, Calvo Jorge, Ocampo-Sosa Alain A
Instituto de Investigación Sanitaria Marqués de Valdecilla (IDIVAL), 39011 Santander, Spain.
Instituto Tecnológico Agrario de Castilla y León (ITACyL), 47071 Valladolid, Spain.
Antibiotics (Basel). 2021 Apr 23;10(5):491. doi: 10.3390/antibiotics10050491.
is one of the most critical bacterial pathogens associated with chronic infections in cystic fibrosis patients. Here we show the phenotypic and genotypic characterization of five consecutive multidrug-resistant isolates of collected during a month from a CF patient with end-stage lung disease and fatal outcome. The isolates exhibited distinct colony morphologies and pigmentation and differences in their capacity to produce biofilm and virulence potential evaluated in larvae of . Whole genome-sequencing showed that isolates belonged to a novel sequence type ST3449 and serotype O6. Analysis of their resistome demonstrated the presence of genes , , , , and and new mutations in chromosomal genes conferring resistance to different antipseudomonal antibiotics. Genes , , , , , and were among the 220 virulence genes detected. The different phenotypic and genotypic features found reveal the adaptation of clone ST3449 to the CF lung environment by a number of mutations affecting genes related with biofilm formation, quorum sensing and antimicrobial resistance. Most of these mutations are commonly found in CF isolates, which may give us important clues for future development of new drug targets to combat chronic infections.
是与囊性纤维化患者慢性感染相关的最关键细菌病原体之一。在此，我们展示了从一名患有终末期肺病且预后不良的囊性纤维化患者在一个月内连续收集的五株多重耐药分离株的表型和基因型特征。这些分离株表现出不同的菌落形态和色素沉着，并且在生物膜形成能力和在幼虫中评估的毒力潜力方面存在差异。全基因组测序表明，分离株属于一种新型序列类型ST3449和血清型O6。对其耐药基因组的分析表明存在基因、、、、和，以及染色体基因中的新突变，这些突变赋予了对不同抗假单胞菌抗生素的抗性。基因、、、、、和在检测到的220个毒力基因中。发现的不同表型和基因型特征揭示了克隆ST3449通过影响与生物膜形成、群体感应和抗菌抗性相关基因的一些突变适应囊性纤维化肺部环境。这些突变中的大多数在囊性纤维化分离株中常见，这可能为未来开发对抗慢性感染的新药物靶点提供重要线索。
