Department of Science & Biotechnology, Faculty of Engineering & Life Sciences, Universiti Selangor, Bestari Jaya 45600, Selangor, Malaysia.
Institute of Bio-IT Selangor, Universiti Selangor, Jalan Zirkon A7/A, Seksyen 7, Shah Alam 40000, Selangor, Malaysia.
Molecules. 2021 Apr 23;26(9):2480. doi: 10.3390/molecules26092480.
The assessment of water-extractable organic matter using an autoclave can provide useful information on physical, chemical, and biological changes within the soil. The present study used virgin forest soils from Chini Forest Reserve, Langkawi Island, and Kenyir Forest Reserve (Malaysia), extracted using different extraction methods. The dissolved organic carbon (DOC), total dissolved nitrogen (TDN), total dissolved phosphorus (TDP), and ammonium-nitrate content were higher in the autoclave treatments, up to 3.0, 1.3, 1.2, and 1.4 times more than by natural extraction (extracted for 24 h at room temperature). Overall, the highest extractable DOC, TDN, TDP, ammonium and nitrate could be seen under autoclaved conditions 121 °C 2×, up to 146.74 mg C/L, 8.97 mg N/L, 0.23 mg P/L, 5.43 mg N mg/L and 3.47 N mg/L, respectively. The soil extracts became slightly acidic with a higher temperature and longer duration. Similar trends were observed in the humic and nonhumic substances, where different types of soil extract treatments influenced the concentrations of the fractions. Different soil extraction methods can provide further details, thus widening the application of soil extracts, especially in microbes.
使用高压灭菌器评估水可提取有机物，可以提供有关土壤内部物理、化学和生物变化的有用信息。本研究使用来自马来西亚珍尼森林保护区、兰卡威岛和肯逸湖森林保护区的原始森林土壤，采用不同的提取方法进行提取。在高压灭菌器处理中，溶解有机碳（DOC）、总溶解氮（TDN）、总溶解磷（TDP）和铵-硝酸盐含量更高，分别比自然提取（在室温下提取 24 小时）高出 3.0、1.3、1.2 和 1.4 倍。总体而言，在 121°C 2×的高压灭菌条件下，可以看到最高的可提取 DOC、TDN、TDP、铵和硝酸盐，分别高达 146.74mg C/L、8.97mg N/L、0.23mg P/L、5.43mg N mg/L 和 3.47N mg/L。土壤提取物的温度升高和时间延长会略微呈酸性。腐殖质和非腐殖质物质也观察到类似的趋势，其中不同类型的土壤提取物处理会影响各部分的浓度。不同的土壤提取方法可以提供更详细的信息，从而拓宽土壤提取物的应用，尤其是在微生物方面。
