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采用不同提取方法从雪兰莪泥炭沼泽和原始森林土壤中获取有价值营养物质的动力学研究及其作为潜在微藻生长促进剂的恢复。

Kinetics Growth and Recovery of Valuable Nutrients from Selangor Peat Swamp and Pristine Forest Soils Using Different Extraction Methods as Potential Microalgae Growth Enhancers.

机构信息

Institute of Bio-IT Selangor, Universiti Selangor, Jalan Zirkon A7/A, Seksyen 7, Shah Alam 40000, Selangor, Malaysia.

Centre for Foundation and General Studies, Universiti Selangor, Jalan Zirkon A7/A, Seksyen 7, Shah Alam 40000, Selangor, Malaysia.

出版信息

Molecules. 2021 Jan 27;26(3):653. doi: 10.3390/molecules26030653.

Abstract

Soil extracts are useful nutrients to enhance the growth of microalgae. Therefore, the present study attempts for the use of virgin soils from Peninsular Malaysia as growth enhancer. Soils collected from Raja Musa Forest Reserve (RMFR) and Ayer Hitam Forest Reserve (AHFR) were treated using different extraction methods. The total dissolved nitrogen (TDN), total dissolved phosphorus (TDP), and dissolved organic carbon (DOC) concentrations in the autoclave methods were relatively higher than natural extraction with up to 132.0 mg N/L, 10.7 mg P/L, and 2629 mg C/L, respectively for RMFR. The results of TDN, TDP, and DOC suggested that the best extraction methods are autoclaved at 121 °C twice with increasing 87%, 84%, and 95%, respectively. TRG 4C dominated the growth at 121 °C twice extraction method in the RMRF and AHRF samples, with increasing 54.3% and 14%, respectively. The specific growth rate (µ) of both microalgae were relatively higher, 0.23 d in the Ayer Hitam Soil. This extract served well as a microalgal growth promoter, reducing the cost and the needs for synthetic medium. Mass production of microalgae as aquatic feed will be attempted eventually. The high recovery rate of nutrients has a huge potential to serve as a growth promoter for microalgae.

摘要

土壤提取物是促进微藻生长的有用养分。因此,本研究尝试使用马来西亚半岛的原始土壤作为生长促进剂。从 Raja Musa 森林保护区 (RMFR) 和 Ayer Hitam 森林保护区 (AHFR) 采集的土壤分别采用不同的提取方法进行处理。高压灭菌方法的总溶解氮 (TDN)、总溶解磷 (TDP) 和溶解有机碳 (DOC) 浓度相对较高,分别为 RMFR 中高达 132.0 mg N/L、10.7 mg P/L 和 2629 mg C/L。TDN、TDP 和 DOC 的结果表明,最佳的提取方法是在 121°C 下高压灭菌两次,分别增加 87%、84%和 95%。在 RMFR 和 AHRF 样品中,TRG 4C 在 121°C 两次提取方法中占主导地位,分别增加了 54.3%和 14%。两种微藻的比生长率(µ)都相对较高,在 Ayer Hitam 土壤中为 0.23 d。该提取物可作为微藻生长促进剂,降低成本和对合成培养基的需求。最终将尝试大规模生产作为水生饲料的微藻。高养分回收率具有作为微藻生长促进剂的巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c07/7866033/0b8a04bbb7d7/molecules-26-00653-g001.jpg

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