Park Miey, Park Eun-Jung, Kim So-Hyeun, Lee Hae-Jeung
Institute for Aging and Clinical Nutrition Research, Gachon University, Gyeonggi-do 13120, Korea.
Department of Food and Nutrition, Gachon University, Gyeonggi-do 13120, Korea.
Int J Mol Sci. 2021 Apr 24;22(9):4444. doi: 10.3390/ijms22094444.
Obesity has become a worldwide health problem, and many significant inflammatory markers have been associated with the risk of side effects of obesity and obesity-related diseases. After a normal diet or high-fat diet with high-fructose water (HFHF) for 8 weeks, male Wistar rats were divided randomly into four experimental groups according to body weight. Next, for 8 weeks, a normal diet, HFHF diet, and HFHF diet with strains ATG-K2 or ATG-K6 were administered orally. Compared to the control group, the HFHF diet group showed significantly increased visceral fat, epididymal fat, and liver weight. The mRNA and protein expression levels of FAS and SREBP-1c were higher in the HFHF diet group than in the HFHF diet with strains ATG-K2 and ATG-K6. The HFHF diet with strain ATG-K2 showed significantly decreased inflammatory cytokine expression in the serum and small intestine compared to the HFHF diet group. Furthermore, histological morphology showed minor cell injury, less severe infiltration, and longer villi height in the small intestine ileum of the HFHF diet with strains groups than in the HFHF diet group. These results suggest that strains K2 and K6 may help reduce intestinal inflammation and could be used as treatment alternatives for intestinal inflammatory reactions and obesity.
肥胖已成为一个全球性的健康问题，许多重要的炎症标志物都与肥胖及其相关疾病的副作用风险有关。雄性Wistar大鼠在正常饮食或高脂高果糖水（HFHF）饮食8周后，根据体重随机分为四个实验组。接下来的8周里，分别给予正常饮食、HFHF饮食以及添加了ATG-K2或ATG-K6菌株的HFHF饮食。与对照组相比，HFHF饮食组的内脏脂肪、附睾脂肪和肝脏重量显著增加。HFHF饮食组中FAS和SREBP-1c的mRNA和蛋白质表达水平高于添加了ATG-K2和ATG-K6菌株的HFHF饮食组。与HFHF饮食组相比，添加了ATG-K2菌株的HFHF饮食组血清和小肠中的炎症细胞因子表达显著降低。此外，组织形态学显示，添加菌株组的HFHF饮食组小肠回肠的细胞损伤较小、浸润较轻且绒毛高度更长。这些结果表明，K2和K6菌株可能有助于减轻肠道炎症，可作为肠道炎症反应和肥胖的治疗替代方案。
