Bazié Wilfried Wenceslas, Goyer Benjamin, Boucher Julien, Zhang Yuwei, Planas Delphine, Chatterjee Debashree, Routy Jean-Pierre, Alary Michel, Ancuta Petronela, Gilbert Caroline
Axe de Recherche Maladies Infectieuses et Immunitaires, CHUL, Centre de Recherche du CHU de Québec-Université Laval, Québec, QC G1V 4G2, Canada.
Programme de Recherche sur les Maladies Infectieuses, Centre Muraz, Institut National de Santé Publique, Bobo-Dioulasso 01 BP 390, Burkina Faso.
Pathogens. 2021 Apr 24;10(5):518. doi: 10.3390/pathogens10050518.
Several types of extracellular vesicles (EVs) secreted by various immune and non-immune cells are present in the human plasma. We previously demonstrated that EV abundance and microRNA content change in pathological conditions, such as HIV infection. Here, we investigated daily variations of large and small EVs, in terms of abundance and microRNA contents in people living with HIV (PLWH) receiving antiretroviral therapy (HIV+ART) and uninfected controls (HIV-).
Venous blood samples from n = 10 HIV+ART and n = 10 HIV- participants were collected at 10:00 and 22:00 the same day. Large and small plasma EVs were purified, counted, and the mature miRNAs miR-29a, miR-29b, miR-92, miR-155, and miR-223 copies were measured by RT-PCR.
Large EVs were significantly bigger in the plasma collected at 10:00 versus 22:00 in both groups. There was a significant day-night increase in the quantity of 5 miRNAs in HIV- large EVs. In HIV+ART, only miR-155 daily variation has been observed in large EVs. Finally, EV-miRNA content permits to distinguish HIV- to HIV+ART in multivariate analysis.
These results point that plasma EV amount and microRNA contents are under daily variation in HIV- people. This new dynamic measure is disrupted in PLWH despite viral-suppressive ART. This study highlights a significant difference concerning EV abundance and their content measured at 22:00 between both groups. Therefore, the time of blood collection must be considered in the future for the EV as biomarkers.
人体血浆中存在多种免疫细胞和非免疫细胞分泌的细胞外囊泡（EVs）。我们之前证明，在诸如HIV感染等病理状态下，EV的丰度和微小RNA含量会发生变化。在此，我们研究了接受抗逆转录病毒治疗的HIV感染者（PLWH）和未感染对照者（HIV-）体内大小EVs在丰度和微小RNA含量方面的每日变化情况。
在同一天的10:00和22:00采集了n = 10名HIV+ART参与者和n = 10名HIV-参与者的静脉血样本。对大小血浆EVs进行纯化、计数，并通过RT-PCR测量成熟微小RNA miR-29a、miR-29b、miR-92、miR-155和miR-223的拷贝数。
两组中，10:00采集的血浆中的大EVs明显比22:00采集的更大。HIV-大EVs中5种微小RNA的数量有显著的昼夜增加。在HIV+ART组中，仅在大EVs中观察到miR-155的每日变化。最后，在多变量分析中，EV-微小RNA含量能够区分HIV-和HIV+ART。
这些结果表明，HIV-人群血浆中EV的数量和微小RNA含量存在每日变化。尽管接受了病毒抑制性抗逆转录病毒治疗，但PLWH人群中的这种新的动态指标仍受到干扰。本研究突出了两组在22:00时EV丰度及其含量的显著差异。因此，未来将血液采集时间纳入考虑对于将EV用作生物标志物很重要。
