Salleh Annas, Zainuddin Zainal Z, Tarmizi Reza M M, Yap Chee K, Jeng Chian-Ren, Zamri-Saad Mohd
Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Borneo Rhino Alliance, c/o Faculty of Sciences and Natural Resources, Universiti Malaysia Sabah, Kota Kinabalu 88400, Sabah, Malaysia.
Animals (Basel). 2021 Apr 20;11(4):1173. doi: 10.3390/ani11041173.
An adult female Sumatran rhinoceros was observed with a swelling in the left infraorbital region in March 2017. The swelling rapidly grew into a mass. A radiograph revealed a cystic radiolucent area in the left maxilla. In June 2017, the rhinoceros was euthanized. At necropsy, the infraorbital mass measured 21 cm × 30 cm. Samples of the infraorbital mass, left parotid gland, and left masseter muscle were collected for histopathology (Hematoxylin & Eosin, Von Kossa, Masson's trichrome, cytokeratin AE1/AE3, EMA, p53, and S-100). Numerous neoplastic epithelial cells showing pleomorphism and infiltration were observed. Islands of dentinoid material containing ghost cells and keratin pearls were observed with the aid of the two special histochemistry stains. Mitotic figures were rarely observed. All the neoplastic odontogenic cells and keratin pearls showed an intense positive stain for cytokeratin AE1/AE3, while some keratin pearls showed mild positive stains for S-100. All samples were negative for p53 and S-100 immunodetection. The mass was diagnosed as a dentinogenic ghost cell tumor.
2017年3月，观察到一头成年雌性苏门答腊犀牛的左眶下区域出现肿胀。该肿胀迅速发展成一个肿块。一张X光片显示左上颌骨有一个囊性透光区。2017年6月，这头犀牛被实施安乐死。尸检时，眶下肿块大小为21厘米×30厘米。采集了眶下肿块、左腮腺和左咬肌的样本用于组织病理学检查（苏木精和伊红染色、冯科萨染色、马森三色染色、细胞角蛋白AE1/AE3、上皮膜抗原、p53和S - 100）。观察到大量呈现多形性和浸润性的肿瘤上皮细胞。借助两种特殊组织化学染色观察到含有影细胞和角化珠的牙本质样物质岛。很少观察到有丝分裂象。所有肿瘤性牙源性细胞和角化珠对细胞角蛋白AE1/AE3呈强阳性染色，而一些角化珠对S - 100呈弱阳性染色。所有样本的p53和S - 100免疫检测均为阴性。该肿块被诊断为牙源性影细胞瘤。
