Ge Qing, Liu Ranlin, Cobine Paul A, Potnis Neha, De La Fuente Leonardo
Department of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, USA.
Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA.
Pathogens. 2021 Apr 20;10(4):495. doi: 10.3390/pathogens10040495.
is a bacterial pathogen causing severe diseases and asymptomatic colonization in more than 600 plants worldwide. Copper (Cu) is a widely used antimicrobial treatment for various plant diseases, including those affecting hosts. Cu homeostasis among strains from different geographical locations and host species has not been characterized. Here, we assessed minimum inhibitory concentration (MIC) of Cu for 54 strains. We observed strain-level variation in MIC values within each subspecies. We hypothesized that these differences could be explained by sequence variation in Cu homeostasis genes. Phylogenies based on , , , and were created using 74 genomes (including 43 strains used in vitro) of , showing that the phylogenetic clustering of Cu homeostasis associated with clustering was based on core genome phylogenies, rather than on pattern of MIC. No association was found among Cu MIC, subspecies classification, and host and location of isolation, probably due to uneven and limited group of strains whose genomes are available. Further analysis focused on a subgroup of isolates from Georgia's vineyards that shared similar Cu-related phenotypes. Further research is needed to better understand the distribution of Cu homeostasis for this pathogen.
是一种细菌病原体，在全球600多种植物中可引起严重疾病和无症状定植。铜（Cu）是一种广泛用于治疗各种植物病害的抗菌剂，包括那些影响宿主的病害。不同地理位置和宿主物种的菌株之间的铜稳态尚未得到表征。在这里，我们评估了54株菌株对铜的最低抑菌浓度（MIC）。我们观察到每个亚种内MIC值存在菌株水平的差异。我们假设这些差异可以通过铜稳态基因的序列变异来解释。使用74个基因组（包括43个体外使用的菌株）构建了基于、、、和的系统发育树，结果表明与聚类相关的铜稳态的系统发育聚类是基于核心基因组系统发育，而不是基于MIC模式。在铜MIC、亚种分类以及分离宿主和地点之间未发现关联，这可能是由于基因组可用的菌株组不均衡且数量有限。进一步的分析集中在来自佐治亚州葡萄园的一组具有相似铜相关表型的分离株上。需要进一步研究以更好地了解该病原体铜稳态的分布情况。
