G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Pr. 100 let Vladivostoku, 690022 Vladivostok, Russia.
Molecules. 2021 Apr 20;26(8):2387. doi: 10.3390/molecules26082387.
We carried out a detailed investigation of PL7 alginate lyases across the genus. The main findings were obtained using the methods of comparative genomics and spatial structure modeling, as well as a phylogenomic approach. Initially, in order to elucidate the alginolytic potential of , we calculated the content of polysaccharide lyase (PL) genes in each genome. The genus-specific PLs were PL1, PL6, PL7 (the most abundant), PL14, PL17, and PL40. We revealed that PL7 belongs to subfamilies 3, 5, and 6. They may be involved in local and horizontal gene transfer and gene duplication processes. Most likely, an individual evolution of PL7 genes promotes the genetic variability of the Alginate Utilization System across . Apparently, the PL7 alginate lyases may acquire a sub-functionalization due to diversification between in-paralogs.
我们对整个属的 PL7 褐藻胶裂解酶进行了详细研究。主要发现是使用比较基因组学和空间结构建模方法以及系统基因组学方法获得的。最初，为了阐明 的 alginolytic 潜力，我们计算了每个基因组中多糖裂解酶 (PL) 基因的含量。属特异性 PL 是 PL1、PL6、PL7（最丰富）、PL14、PL17 和 PL40。我们揭示了 PL7 属于亚家族 3、5 和 6。它们可能参与局部和水平基因转移和基因复制过程。很可能，PL7 基因的个体进化促进了 Alginate Utilization System 在 中的遗传多样性。显然，PL7 褐藻胶裂解酶可能由于 in-paralogs 之间的多样化而获得次功能化。
