Dries Leonie, Bussotti Simone, Pozzi Carlo, Kunz Robert, Schnell Sylvia, Löhnertz Otmar, Vortkamp Anne
Department of Soil Science and Plant Nutrition, Hochschule Geisenheim University, 65366 Geisenheim, Germany.
Department of Agricultural and Environmental Sciences, University of Milano, 20133 Milano, Italy.
Microorganisms. 2021 Apr 13;9(4):822. doi: 10.3390/microorganisms9040822.
The microbiota associated with the rhizosphere is responsible for crucial processes. Understanding how the plant and its bacterial community interact is of great importance to face the upcoming agricultural and viticultural challenges. The composition of the bacterial communities associated with the rhizosphere of grapevines is the result of the interaction between many drivers: biogeography, edaphic factors, soil management and plant genotype. The experimental design of this study aimed to reduce the variability resulting from all factors except the genotype of the rootstock. This was made possible by investigating four ungrafted grapevine rootstock varieties of the same age, grown on the same soil under the same climatic conditions and managed identically. The bacterial communities associated with the rhizosphere of the rootstocks 1103 Paulsen, 140 Ruggeri, 161-49 Couderc and Kober 5BB were characterized with the amplicon based sequencing technique, targeting regions V4-V5 of 16S rRNA gene. Linear discriminant analysis effect Size (LEfSe) analysis was performed to determine differential abundant taxa. The four rootstocks showed similarities concerning the structure of the bacteria assemblage (richness and evenness). Nonetheless, differences were detected in the composition of the bacterial communities. Indeed, all investigated rootstocks recruited communities with distinguishable traits, thus confirming the role of rootstock genotype as driver of the bacteria composition.
与根际相关的微生物群负责关键过程。了解植物与其细菌群落如何相互作用对于应对即将到来的农业和葡萄栽培挑战至关重要。与葡萄树根际相关的细菌群落组成是多种驱动因素相互作用的结果：生物地理学、土壤因素、土壤管理和植物基因型。本研究的实验设计旨在减少除砧木基因型外所有因素导致的变异性。通过研究四个同龄的未嫁接葡萄砧木品种得以实现这一点，这些品种种植在相同土壤上，处于相同气候条件下且管理方式相同。利用基于扩增子的测序技术，针对16S rRNA基因的V4 - V5区域，对与砧木1103 Paulsen、140 Ruggeri、161 - 49 Couderc和Kober 5BB根际相关的细菌群落进行了表征。进行线性判别分析效应大小（LEfSe）分析以确定差异丰富的分类群。这四种砧木在细菌组合结构（丰富度和均匀度）方面表现出相似性。尽管如此，在细菌群落组成中仍检测到差异。事实上，所有研究的砧木都招募了具有可区分特征的群落，从而证实了砧木基因型作为细菌组成驱动因素的作用。
