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不同乳酸菌菌株发酵巴氏杀菌木瓜泥对消化过程中酚类化合物质量和生物可及性的影响

Influence of Fermentation of Pasteurised Papaya Puree with Different Lactic Acid Bacterial Strains on Quality and Bioaccessibility of Phenolic Compounds during Digestion.

作者信息

Mashitoa Florence M, Akinola Stephen A, Manhevi Vimbainashe E, Garcia Cyrielle, Remize Fabienne, Slabbert Retha M, Sivakumar Dharini

机构信息

Department of Horticulture, Tshwane University of Technology, Pretoria West 0001, South Africa.

Phytochemical Food Network Group, Department of Crop Sciences, Pretoria West 0001, South Africa.

出版信息

Foods. 2021 Apr 28;10(5):962. doi: 10.3390/foods10050962.

Abstract

This study describes the impact of utilising different strains of lactic acid bacteria (LAB) for the fermentation of papaya puree and their effect on the quality parameters and bioaccessibility of phenolic compounds during simulated gastrointestinal digestion. Papaya was processed into puree; pasteurised and fermented at 37 °C for 2 days; and stored for 7 days at 4 °C using LAB strains (; ), (; ) and (; ), respectively. Non-fermented samples at 0 (), 2 () and 7 days () served as controls. pH was reduced with fermentation and was lowest in (3.03) and (3.16) after storage. The colour change () increased with the fermentation and storage of purees; showed the highest (13.8), and its sourness reduced with storage. The fermentation by and increased the % recovery of chlorogenic, vanillic, syringic, ellagic, ferulic acids, catechin, epicatechin and quercetin in the intestinal fraction compared to the and . Fermentation by and significantly improved the antioxidant capacity of the dialysed fraction compared to the or . -fermented papaya puree showed the highest inhibitory effect of α-glucosidase activity followed by . had a significantly higher survival rate. LAB fermentation affected the bioacessibilities of phenolics and was strain dependent. This study recommends the use of for fermenting papaya puree.

摘要

本研究描述了利用不同乳酸菌菌株发酵木瓜泥的影响,以及在模拟胃肠道消化过程中它们对酚类化合物质量参数和生物可及性的影响。将木瓜加工成泥;巴氏杀菌后在37℃发酵2天;并分别使用乳酸菌菌株(;)、(;)和(;)在4℃储存7天。0天()、2天()和7天()的未发酵样品作为对照。发酵后pH值降低,储存后在(3.03)和(3.16)中最低。随着泥的发酵和储存,颜色变化()增加;显示出最高的(13.8),并且其酸度随着储存而降低。与和相比,和的发酵增加了肠道部分中绿原酸、香草酸、丁香酸、鞣花酸、阿魏酸、儿茶素、表儿茶素和槲皮素的回收率。与或相比,和的发酵显著提高了透析部分的抗氧化能力。发酵的木瓜泥对α-葡萄糖苷酶活性的抑制作用最高,其次是。的存活率显著更高。乳酸菌发酵影响酚类化合物的生物可及性,且具有菌株依赖性。本研究推荐使用发酵木瓜泥。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ce/8145966/cb830a93c6c0/foods-10-00962-g001.jpg

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