Department of Biotechnology and Food Technology, Southern Taiwan University of Science and Technology, Tainan, Taiwan.
Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
Bioresour Technol. 2020 Jul;308:123266. doi: 10.1016/j.biortech.2020.123266. Epub 2020 Mar 28.
Lactic acid (LA) fermentation was conducted with suspended and immobilized cells of an isolated Lactobacillus plantarum 23 strain using various fermentation strategies. Glucose and an alternative, relatively inexpensive carbon source - the hydrolysate of microalga Chlorella vulgaris ESP-31, were used as the carbon source. Batch fermentation using immobilized cells of L. plantarum 23 could enhance LA titer and yield by 43% and 39%, respectively, when compared with the suspended culture. Fed-batch culture integrated with in situ LA removal via ion exchange raised LA productivity by 72% by overcoming product inhibition. The highest LA productivity from glucose with PVA immobilized cells was 14.22 g/L/h, achieved under continuous operation at 50% w/v loading of immobilized beads and hydraulic retention time (HRT) of 2 h. PVA immobilized L. plantarum 23 could also use microalgal hydrolysate as the renewable carbon source, and the highest LA productivity was 9.93 g/L/h under continuous fermentation at 4 h HRT.
乳酸（LA）发酵采用悬浮和固定化的植物乳杆菌 23 菌株细胞，使用各种发酵策略。葡萄糖和替代的、相对便宜的碳源-小球藻 ESP-31 的水解物被用作碳源。与悬浮培养相比，使用固定化植物乳杆菌 23 细胞的分批发酵可以分别将 LA 滴度和产率提高 43%和 39%。通过离子交换原位去除 LA 的补料分批培养通过克服产物抑制将 LA 生产率提高了 72%。在 50%（w/v）固定珠载量和 2 h 水力停留时间（HRT）下进行连续操作时，用 PVA 固定化细胞从葡萄糖获得的最高 LA 生产率为 14.22 g/L/h。PVA 固定化植物乳杆菌 23 也可以使用微藻水解物作为可再生碳源，在 4 h HRT 的连续发酵下，最高 LA 生产率为 9.93 g/L/h。
