Rey Federica, Messa Letizia, Pandini Cecilia, Maghraby Erika, Barzaghini Bianca, Garofalo Maria, Micheletto Giancarlo, Raimondi Manuela Teresa, Bertoli Simona, Cereda Cristina, Zuccotti Gian Vincenzo, Cancello Raffaella, Carelli Stephana
Department of Biomedical and Clinical Sciences "L. Sacco", School of Medicine, University of Milan, Via G.B. Grassi 74, 20157 Milan, Italy.
Paediatric Clinical Research Center Fondazione "Romeo ed Enrica Invernizzi", University of Milan, Via G.B. Grassi 74, 20157 Milan, Italy.
J Pers Med. 2021 Apr 28;11(5):352. doi: 10.3390/jpm11050352.
Obesity is a multifactorial disease presenting sex-related differences including adipocyte functions, sex hormone effects, genetics, and metabolic inflammation. These can influence individuals' risk for metabolic dysfunctions, with an urgent need to perform sex-based analysis to improve prevention, treatment, and rehabilitation programs. This research work is aimed at characterizing the transcriptional differences present in subcutaneous adipose tissue (SAT) of five obesity affected men versus five obesity affected women, with an additional focus on the role of long non-coding RNAs. Through RNA-sequencing, we highlighted the presence of both coding and non-coding differentially expressed RNAs, and with numerous computational analyses we identified the processes in which these genes are implicated, along with their role in co-morbidities development. We report 51 differentially expressed transcripts, 32 of which were coding genes and 19 were non-coding. Using the WGCNA R package (Weighted Correlation Network Analysis, version 1.70-3), we describe the interactions between coding and non-coding RNAs, and the non-coding RNAs association with the insurgence of specific diseases, such as cancer development, neurodegenerative diseases, and schizophrenia. In conclusion, our work highlights a specific gender sex-related transcriptional signature in the SAT of obesity affected patients.
肥胖是一种多因素疾病，存在与性别相关的差异，包括脂肪细胞功能、性激素作用、遗传学和代谢炎症。这些因素会影响个体发生代谢功能障碍的风险，因此迫切需要进行基于性别的分析，以改进预防、治疗和康复方案。这项研究旨在表征五名肥胖男性与五名肥胖女性的皮下脂肪组织（SAT）中存在的转录差异，并特别关注长链非编码RNA的作用。通过RNA测序，我们突出了编码和非编码差异表达RNA的存在，并通过大量计算分析确定了这些基因所涉及的过程，以及它们在共病发展中的作用。我们报告了51个差异表达的转录本，其中32个是编码基因，19个是非编码基因。使用WGCNA R包（加权相关网络分析，版本1.70 - 3），我们描述了编码和非编码RNA之间的相互作用，以及非编码RNA与特定疾病（如癌症发展、神经退行性疾病和精神分裂症）发生的关联。总之，我们的工作突出了肥胖患者SAT中一种特定的性别相关转录特征。
