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水杨酸和茉莉酸甲酯处理下货架期条件下接种的杏果实物理和抗氧化特性及酶活性的时间模式和相互关系

Temporal Patterns and Inter-Correlations among Physical and Antioxidant Attributes and Enzyme Activities of Apricot Fruit Inoculated with under Salicylic Acid and Methyl Jasmonate Treatments under Shelf-Life Conditions.

作者信息

Ezzat Ahmed, Szabó Szilárd, Szabó Zoltán, Hegedűs Attila, Berényi Dorina, Holb Imre J

机构信息

Department of Horticulture, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Shaikh 33516, Egypt.

Department of Physical Geography and Geoinformatics, University of Debrecen, H-4032 Debrecen, Hungary.

出版信息

J Fungi (Basel). 2021 Apr 28;7(5):341. doi: 10.3390/jof7050341.

Abstract

causes serious postharvest damage on apricot fruits under shelf-life storage conditions. Plant elicitors of methyl jasmonate (MeJA) and salicylic acid (SA) can reduce this damage, and their research can explain the background of the plant defense physiological processes in -infected fruits. The aims of this study were: (i) to evaluate the effect of various concentrations of MeJA and SA on brown rot incidence (BRI) and lesion diameter (LD) of apricot fruits; (ii) to measure the temporal patterns for the effect of 0.4 mmol L MeJA and 2 mmol L SA treatments on BRI, LD and seven fruit measures (fruit firmness (FF), lignin content (LC), total soluble phenol content (TSPC), total antioxidant capacity (TAC) and enzyme activities of PAL, POD and SOD) in treatments of -inoculated versus (vs.) non-inoculated fruits over an eight-day shelf-life storage period; and (iii) to determine inter-correlations among the seven fruit measures for MeJA and SA treatments. Both MeJA and SA significantly reduced BRI and LD. LC, FF, TAC, TSPC, as well as SOD and PAL activities in the MeJA and SA treatments were higher than the water-treated control in most assessment days and both inoculation treatments. In both inoculation treatments, the activity of POD in the SA-treated fruits was higher than MeJA-treated and control fruits at all dates. In MeJA vs. SA and inoculated vs. non-inoculated treatments, six variable pairs (FF vs. TSPC, FF vs. TAC, TAC vs. PAL, PAL vs. POD, PAL vs. SOD, and POD vs. SOD) showed significant inter-correlation values. Principal component analyses explained 96% and 93% of the total variance for inoculated and non-inoculated treatments, respectively. In inoculated treatments, both PC1 and PC2 explained 41% of the total variance and correlated with FF, TSPC and TAC and with PAL, SOD and POD, respectively. In non-inoculated treatments, PC1 and PC2 explained 49% and 44% of the total variance and correlated with LC, PAL, POD and SOD and with FF, TSPC and TAC, respectively. It can be concluded that MeJA and SA are useful in the practice to enhance the plant defense system against brown rot by reducing fungal growth and by improving physical and antioxidant attributes (FF, LC, TAC and TSPC) and the activity of defense-related enzymes (PAL, POD and SOD) in apricot fruits during shelf-life storage conditions.

摘要

在货架期储存条件下,会对杏果实造成严重的采后损伤。茉莉酸甲酯(MeJA)和水杨酸(SA)等植物激发子可以减少这种损伤,对它们的研究能够解释受感染果实中植物防御生理过程的背景。本研究的目的是:(i)评估不同浓度的MeJA和SA对杏果实褐腐病发病率(BRI)和病斑直径(LD)的影响;(ii)测定在八天的货架期储存期间,0.4 mmol/L MeJA和2 mmol/L SA处理对接种和未接种果实的BRI、LD以及七个果实指标(果实硬度(FF)、木质素含量(LC)、总可溶性酚含量(TSPC)、总抗氧化能力(TAC)以及苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和超氧化物歧化酶(SOD)的酶活性)的影响的时间模式;(iii)确定MeJA和SA处理的七个果实指标之间的相互关系。MeJA和SA均显著降低了BRI和LD。在大多数评估日以及两种接种处理中,MeJA和SA处理中的LC、FF、TAC、TSPC以及SOD和PAL活性均高于水处理对照。在两种接种处理中,SA处理果实中POD的活性在所有日期均高于MeJA处理和对照果实。在MeJA与SA以及接种与未接种处理中,六对变量(FF与TSPC、FF与TAC、TAC与PAL、PAL与POD、PAL与SOD以及POD与SOD)显示出显著的相互关系值。主成分分析分别解释了接种和未接种处理总方差的96%和93%。在接种处理中,PC1和PC2均解释了总方差的41%,分别与FF、TSPC和TAC以及PAL、SOD和POD相关。在未接种处理中,PC1和PC2分别解释了总方差的49%和44%,分别与LC、PAL、POD和SOD以及FF、TSPC和TAC相关。可以得出结论,在货架期储存条件下,MeJA和SA在实践中有助于增强植物防御系统以抵御褐腐病,通过减少真菌生长以及改善杏果实的物理和抗氧化特性(FF、LC、TAC和TSPC)以及防御相关酶(PAL、POD和SOD)的活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f3e/8145973/e1857a3f926e/jof-07-00341-g001.jpg

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