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GmARF15通过响应水杨酸信号促进 表达来增强大豆对 的抗性。 （注：原文中“by Promoting Expression”和“to ”处有缺失内容）

GmARF15 Enhances the Resistance of Soybean to by Promoting Expression in Response to Salicylic Acid Signalling.

作者信息

Huo Yuhan, Chen Haiyuan, Zhang Zhuo, Song Yang, Liu Siyan, Wang Piwu, Fan Sujie

机构信息

Plant Biotechnology Center, College of Agronomy, Jilin Agriculture University, Changchun 130118, China.

出版信息

Int J Mol Sci. 2024 Dec 29;26(1):191. doi: 10.3390/ijms26010191.

DOI:10.3390/ijms26010191
PMID:39796049
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11720048/
Abstract

Phytophthora root and stem rot caused by () is a globally prevalent oomycete disease. The use of resistant cultivars is an effective and environmentally friendly strategy to manage this disease. It is important to understand the molecular mechanisms underlying the response of (soybean) to infection. In this study, we demonstrated that an isoflavonoid-specific prenyltransferase gene (, Glyma.10G070300) was significantly upregulated in the soybean cultivar Williams 82 with high resistance to infection. Transgenic soybean seedlings overexpressing exhibited enhanced resistance to , and those subjected to RNA interference showed increased susceptibility to the pathogen. Yeast-one-hybrid and electrophoretic mobility shift assays revealed that GmARF15 could directly bind to the promoter of . Further analysis of the function showed that transgenic soybean seedlings overexpressing also exhibited enhanced resistance to . Transactivation assay, luciferase assay, and qPCR analysis showed that GmARF15 could promote the expression of . Further analysis indicated that elevated salicylic acid levels were associated with increased expression of and . Taken together, these findings reveal a regulatory mechanism by which GmARF15 enhances soybean resistance to . , potentially by promoting the expression of through the salicylic acid signaling pathway.

摘要

由（）引起的疫霉根腐病和茎腐病是一种全球普遍存在的卵菌病害。使用抗性品种是防治这种病害的一种有效且环保的策略。了解（大豆）对感染反应的分子机制很重要。在本研究中，我们证明了一个异黄酮特异性异戊烯基转移酶基因（，Glyma.10G070300）在对感染具有高抗性的大豆品种Williams 82中显著上调。过表达的转基因大豆幼苗对表现出增强的抗性，而那些进行RNA干扰的幼苗对病原体的易感性增加。酵母单杂交和电泳迁移率变动分析表明，GmARF15可以直接结合到的启动子上。对功能的进一步分析表明，过表达的转基因大豆幼苗对也表现出增强的抗性。反式激活测定、荧光素酶测定和qPCR分析表明，GmARF15可以促进的表达。进一步分析表明，水杨酸水平升高与和的表达增加有关。综上所述，这些发现揭示了一种调控机制，通过该机制GmARF15增强大豆对的抗性，可能是通过水杨酸信号通路促进的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/4b8d13d54a79/ijms-26-00191-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/e8151a16a8fc/ijms-26-00191-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/fd296654b74a/ijms-26-00191-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/b2201257ca01/ijms-26-00191-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/a98c6d7d717f/ijms-26-00191-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/4b8d13d54a79/ijms-26-00191-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/e8151a16a8fc/ijms-26-00191-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/dd89b0e28920/ijms-26-00191-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/cf5d063dea5a/ijms-26-00191-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/fd296654b74a/ijms-26-00191-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d17e/11720048/4b8d13d54a79/ijms-26-00191-g007.jpg

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