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在含细胞分裂素培养基和渗透胁迫下诱导的马铃薯微型薯的基因表达分析

Gene Expression Analysis of Microtubers of Potato L. Induced in Cytokinin Containing Medium and Osmotic Stress.

作者信息

Herrera-Isidron Lisset, Valencia-Lozano Eliana, Rosiles-Loeza Pablo Yamild, Robles-Hernández Maria Guadalupe, Napsuciale-Heredia Abigail, Cabrera-Ponce Jose Luis

机构信息

Unidad Profesional Interdisciplinaria de Ingeniería Campus Guanajuato (UPIIG), Instituto Politécnico Nacional, Av. Mineral de Valenciana 200, Puerto Interior, Silao de la Victoria, Guanajuato 36275, Mexico.

Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato 36824, Mexico.

出版信息

Plants (Basel). 2021 Apr 27;10(5):876. doi: 10.3390/plants10050876.

DOI:10.3390/plants10050876
PMID:33925316
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8146008/
Abstract

Potato microtuber productions through in vitro techniques are ideal propagules for producing high quality seed potatoes. Microtuber development is influenced by several factors, i.e., high content sucrose and cytokinins are among them. To understand a molecular mechanism of microtuberization using osmotic stress and cytokinin signaling will help us to elucidate this process. We demonstrate in this work a rapid and efficient protocol for microtuber development and gene expression analysis. Medium with high content of sucrose and gelrite supplemented with 2iP as cytokinin under darkness condition produced the higher quantity and quality of microtubers. Gene expression analysis of genes involved in the two-component signaling system (), cytokinin signaling, (, , ) homeodomains (, , ), auxin signaling, , carbon metabolism (, , protein synthesis, and a morphogenetic regulator of tuberization () was performed by qPCR real time. Differential gene expression was observed during microtuber development. Gene regulation of two component and cytokinin signaling is taking place during this developmental process, yielding more microtubers. Further analysis of each component is required to elucidate it.

摘要

通过体外技术生产马铃薯微型薯是生产高质量种薯的理想繁殖体。微型薯的发育受多种因素影响，其中高含量蔗糖和细胞分裂素是影响因素之一。利用渗透胁迫和细胞分裂素信号传导来理解微型薯形成的分子机制将有助于我们阐明这一过程。我们在这项工作中展示了一种用于微型薯发育和基因表达分析的快速高效方案。在黑暗条件下，含有高含量蔗糖和添加了2iP作为细胞分裂素的结冷胶的培养基产生了数量更多、质量更高的微型薯。通过实时定量PCR对参与双组分信号系统（）、细胞分裂素信号传导（、、）、同源结构域（、、）、生长素信号传导（）、碳代谢（、、蛋白质合成）以及块茎形成的形态发生调节因子（）的基因进行了表达分析。在微型薯发育过程中观察到了基因表达差异。在这个发育过程中发生了双组分和细胞分裂素信号传导的基因调控，从而产生了更多的微型薯。需要对每个组分进行进一步分析以阐明这一过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/966a/8146008/f04c3d5f15e7/plants-10-00876-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/966a/8146008/058ea5eb0a43/plants-10-00876-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/966a/8146008/79f468cc922f/plants-10-00876-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/966a/8146008/613af02a63b5/plants-10-00876-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/966a/8146008/f04c3d5f15e7/plants-10-00876-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/966a/8146008/058ea5eb0a43/plants-10-00876-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/966a/8146008/79f468cc922f/plants-10-00876-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/966a/8146008/613af02a63b5/plants-10-00876-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/966a/8146008/f04c3d5f15e7/plants-10-00876-g004.jpg

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The Role of Mechanoperception in Plant Cell Wall Integrity Maintenance.机械感知在植物细胞壁完整性维持中的作用
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An integrative overview of physiological and proteomic changes of cytokinin-induced potato (Solanum tuberosum L.) tuber development in vitro.
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