Ph.D. Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei 11031, Taiwan.
Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan.
Curr Issues Mol Biol. 2021 Apr 27;43(1):2-20. doi: 10.3390/cimb43010002.
Colorectal cancer (CRC) has the fourth-highest incidence of all cancer types, and its incidence has steadily increased in the last decade. The general transcription factor III (GTF3) family, comprising GTF3A, GTF3B, GTF3C1, and GTFC2, were stated to be linked with the expansion of different types of cancers; however, their messenger (m)RNA expressions and prognostic values in colorectal cancer need to be further investigated. To study the transcriptomic expression levels of GTF3 gene members in colorectal cancer in both cancerous tissues and cell lines, we first performed high-throughput screening using the Oncomine, GEPIA, and CCLE databases. We then applied the Prognoscan database to query correlations of their mRNA expressions with the disease-specific survival (DSS), overall survival (OS), and disease-free survival (DFS) status of the colorectal cancer patient. Furthermore, proteomics expressions of GTF3 family members in clinical colorectal cancer specimens were also examined using the Human Protein Atlas. Finally, genomic alterations of GTF3 family gene expressions in colorectal cancer and their signal transduction pathways were studied using cBioPortal, ClueGO, CluePedia, and MetaCore platform. Our findings revealed that GTF3 family members' expressions were significantly correlated with the cell cycle, oxidative stress, WNT/β-catenin signaling, Rho GTPases, and G-protein-coupled receptors (GPCRs). Clinically, high GTF3A and GTF3B expressions were significantly correlated with poor prognoses in colorectal cancer patients. Collectively, our study declares that GTF3A was overexpressed in cancer tissues and cell lines, particularly colorectal cancer, and it could possibly step in as a potential prognostic biomarker.
结直肠癌（CRC）是所有癌症类型中发病率第四高的癌症，在过去十年中其发病率稳步上升。一般转录因子 III（GTF3）家族，包括 GTF3A、GTF3B、GTF3C1 和 GTFC2，被认为与不同类型癌症的扩张有关；然而，它们在结直肠癌中的信使（m）RNA 表达和预后价值需要进一步研究。为了研究 GTF3 基因成员在结直肠癌组织和细胞系中的转录组表达水平，我们首先使用 Oncomine、GEPIA 和 CCLE 数据库进行高通量筛选。然后，我们应用 Prognoscan 数据库查询它们的 mRNA 表达与结直肠癌患者的疾病特异性生存（DSS）、总生存（OS）和无病生存（DFS）状态的相关性。此外，还使用人类蛋白质图谱检查了临床结直肠癌标本中 GTF3 家族成员的蛋白质组学表达。最后，使用 cBioPortal、ClueGO、CluePedia 和 MetaCore 平台研究了结直肠癌中 GTF3 家族基因表达的基因组改变及其信号转导途径。我们的研究结果表明，GTF3 家族成员的表达与细胞周期、氧化应激、WNT/β-catenin 信号、Rho GTPases 和 G 蛋白偶联受体（GPCRs）显著相关。临床上，高 GTF3A 和 GTF3B 表达与结直肠癌患者的不良预后显著相关。总的来说，我们的研究表明 GTF3A 在癌症组织和细胞系中过度表达，特别是在结直肠癌中，它可能成为一个潜在的预后生物标志物。
