Department of Pathology and Urology, Faculty of Medicine, Assiut University, Assiut 71111, Egypt.
Department of Urology, Faculty of Medicine, Assiut University, Assiut 71111, Egypt.
Cells. 2021 Apr 27;10(5):1029. doi: 10.3390/cells10051029.
The histopathologic diagnosis of prostate cancer (PCa) from biopsies is a current challenge if double or triple staining is needed. Therefore, there is an urgent need for development of a new reliable biomarker to diagnose PCa patients. We aimed to explore and compare the expression of TMTC4 in PCa cells and tissue specimens and evaluate its sensitivity and specificity. The expression of TMTC4 in PCa and normal prostate epithelial cells was determined by real-time PCR and Western blot analyses. Immunohistochemical (IHC) staining of TMTC4 was performed on tissues collected from PCa and benign prostatic hyperplasia (BPH). Our results show a high expression of TMTC4 on mRNA and protein levels in PCa versus BPH1 and normal cells ( < 0.05). IHC results show strong cytoplasmic expressions in PCa cases ( < 0.001) as compared to BPH cases. The overall accuracy as measured by the AUC was 1.0 ( < 0.001). The sensitivity and specificity of the protein were 100% and 96.6%, respectively. Taken together, we report a high TMTC4 expression in PCa cells and tissues and its ability to differentiate between PCa and BPH with high sensitivity and specificity. This finding can be carried over to clinical practice after its confirmation by further studies.
前列腺癌（PCa）活检的组织病理学诊断是当前的一个挑战，如果需要双重或三重染色的话。因此，迫切需要开发一种新的可靠的生物标志物来诊断 PCa 患者。我们旨在探索和比较 TMTC4 在 PCa 细胞和组织标本中的表达，并评估其敏感性和特异性。通过实时 PCR 和 Western blot 分析确定 TMTC4 在 PCa 和正常前列腺上皮细胞中的表达。对来自 PCa 和良性前列腺增生（BPH）的组织进行 TMTC4 的免疫组织化学（IHC）染色。我们的结果表明，与 BPH1 和正常细胞相比，TMTC4 在 PCa 中的 mRNA 和蛋白水平表达较高（<0.05）。与 BPH 病例相比，IHC 结果显示 PCa 病例中存在强烈的细胞质表达（<0.001）。AUC 测量的总体准确性为 1.0（<0.001）。该蛋白的灵敏度和特异性分别为 100%和 96.6%。总之，我们报告了 TMTC4 在 PCa 细胞和组织中的高表达，以及它具有高灵敏度和特异性来区分 PCa 和 BPH 的能力。这一发现可以在进一步研究确认后应用于临床实践。
