He Hui-chan, Bi Xue-cheng, Zheng Zhi-wei, Dai Qi-shan, Han Zhao-Dong, Liang Yu-Xiang, Ye Yong-Kang, Zeng Guo-hua, Zhu Gang, Zhong Wei-de
Guangzhou First Municipal People's Hospital, Affiliated Guangzhou Medical College, Guangzhou 510180, China.
Med Oncol. 2009;26(3):303-8. doi: 10.1007/s12032-008-9120-9. Epub 2008 Nov 12.
To investigate the expressions of PIM-1 and hK2 mRNA in normal prostate, benign prostatic glandular hyperplasia (BPH), and prostate cancer (PCa), and to explore the association of PIM-1 and hK2 expressions with PCa progression. The samples were harvested from 37 patients with BPH, 23 patients with PCa, and three with normal prostate tissues. Total RNA was extracted from their prostate tissues and analyzed for PIM-1 and hK2 mRNA levels using SYBR green I-based quantitative real-time RT-PCR (QRT-PCR) assays and Southern blot analysis. The differences of gene expressions were calculated based on standard curve. Quantitative expressions of PIM-1 and hK2 mRNA in normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74, 4.45 +/- 0.63, and 1.02 +/- 0.03, 2.264 +/- 0.46, 5.905 +/- 0.78, respectively. PIM-1 and hK2 were expressed higher in PCa than those in BPH and normal prostate tissues, the differences among which had statistic significance (P < 0.05). Our results support the hypothesis that PIM-1 and hK2 play a significant role in the growth of PCa and the detection of PIM-1 and hK2 mRNA expressions by QRT-PCR provided more reliable and helpful information on diagnosis, treatment, and prognosis of PCa.
研究PIM-1和hK2 mRNA在正常前列腺组织、良性前列腺增生（BPH）及前列腺癌（PCa）中的表达情况，并探讨PIM-1和hK2表达与PCa进展的相关性。收集了37例BPH患者、23例PCa患者及3例正常前列腺组织患者的样本。从其前列腺组织中提取总RNA，采用基于SYBR green I的定量实时逆转录聚合酶链反应（QRT-PCR）检测及Southern印迹分析检测PIM-1和hK2 mRNA水平。根据标准曲线计算基因表达差异。正常前列腺组织、BPH及PCa中PIM-1和hK2 mRNA的定量表达分别为1.05±0.04、2.57±0.74、4.45±0.63及1.02±0.03、2.264±0.46、5.905±0.78。PIM-1和hK2在PCa中的表达高于BPH及正常前列腺组织，差异具有统计学意义（P<0.05）。我们的结果支持以下假设：PIM-1和hK2在PCa生长中起重要作用，通过QRT-PCR检测PIM-1和hK2 mRNA表达可为PCa的诊断、治疗及预后提供更可靠且有用的信息。
