Meneguzzi Mariana, Pissetti Caroline, Rebelatto Raquel, Trachsel Julian, Kuchiishi Suzana Satomi, Reis Adrienny Trindade, Guedes Roberto Maurício Carvalho, Leão Joice Aparecida, Reichen Caroline, Kich Jalusa Deon
Curso de Medicina Veterinária, Instituto Federal Catarinense, Concórdia 89703-720, SC, Brazil.
Departamento de Medicina Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre 91540-000, RS, Brazil.
Microorganisms. 2021 Apr 27;9(5):947. doi: 10.3390/microorganisms9050947.
Clinical salmonellosis has been increasing significantly in Brazil in recent years. A total of 130 outbreaks distributed among 10 swine-producing states were investigated. One representative isolate from each outbreak was characterized through serotyping, antimicrobial resistance profiles, PFGE, and WGS. From 130 outbreaks: 50 were enteric, 48 were septicemic, 17 cases were characterized as hepato-biliary invasive, 13 as nodal and two were not classified. The most prevalent serovars were a monophasic variant of (55/130), Choleraesuis (46/130), and Typhimurium (14/130). Most of the strains (86.92%) demonstrated a high rate of multi-drug resistance. The identification of a major Choleraesuis clonal group in several Brazilian states sharing the same resistance genes suggested that these strains were closely related. Six strains from this clonal group were sequenced, revealing the same ST-145 and 11 to 47 different SNPs. The detected plasmid type showed multiple marker genes as RepA_1_pKPC-CAV1321, the first to be reported in . All AMR genes detected in the genomes were likely present on plasmids, and their phenotype was related to genotypic resistance genes. These findings reveal that salmonellosis is endemic in the most important pig-producing states in Brazil, emphasizing the need to make data available to aid in reducing its occurrence.
近年来，巴西临床沙门氏菌病显著增加。对分布在10个生猪生产州的130起疫情进行了调查。从每起疫情中选取一株代表性分离株，通过血清分型、抗菌药物耐药谱、脉冲场凝胶电泳（PFGE）和全基因组测序（WGS）进行特征分析。在130起疫情中：50起为肠道型，48起为败血症型，17例为肝-胆侵袭型，13例为淋巴结型，2例未分类。最常见的血清型是单相变异型（55/130）、猪霍乱沙门氏菌（46/130）和鼠伤寒沙门氏菌（14/130）。大多数菌株（86.92%）表现出高度的多重耐药性。在巴西几个州鉴定出一个主要的猪霍乱沙门氏菌克隆群，它们具有相同的耐药基因，这表明这些菌株密切相关。对该克隆群的6株菌株进行测序，结果显示相同的ST-145以及11至47个不同的单核苷酸多态性（SNP）。检测到的质粒类型显示出多个标记基因，如RepA_1_pKPC-CAV1321，这是在[具体范围未提及]首次报道的。在基因组中检测到的所有抗菌药物耐药（AMR）基因可能都存在于质粒上，并且它们的表型与基因型耐药基因相关。这些发现表明，沙门氏菌病在巴西最重要的生猪生产州呈地方流行，强调了提供数据以帮助减少其发生的必要性。
